Positive control:
Lane 1: Rat kidney tissue lysate
Lane 2: Human liver tissue lysate
Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
ACY1 Mouse Monoclonal Antibody [15G2] (EM1901-86)
Immunogen
Recombinant protein within human acy1 aa 50-250.
Host
Mouse
Positive Control
Rat kidney tissue lysate, human liver tissue lysate, Rat liver tissue, human liver tissue, human kidney tissue, mouse small intestine tissue, SHSY5Y.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
15G2
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
46 kDa
Isotype
IgG2a
APPLICATION DILUTION
-
WB
-
1:500-1:1000
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
ACY1
SYNONYMS
ACY 1 antibody; ACY-1 antibody; Acy1 antibody; ACY1_HUMAN antibody; ACY1D antibody; ACYLASE antibody; Acylase I antibody; Aminoacylase 1 antibody; Aminoacylase-1 antibody; EC 3.5.1.14 antibody; epididymis secretory protein Li 5 antibody; HEL-S-5 antibody; N acyl L amino acid amidohydrolase antibody; N-acyl-L-amino-acid amidohydrolase antibody; OTTHUMP00000212459 antibody; OTTHUMP00000212462 antibody; OTTHUMP00000212463 antibody; OTTHUMP00000212464 antibody; OTTHUMP00000212465 antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase M20A family.
TISSUE SPECIFICITY
Expression is highest in kidney, strong in brain and weaker in placenta and spleen.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Aminoacylase is involved in the regulation of the urea cycle. N-acetyl-L-glutamate is an allosteric activator of carbamoyl phosphate synthetase, a crucial enzyme that commits NH4+ molecules to the urea cycle. The urea cycle gets rid of excess ammonia (NH4+) in the body, a process that must be up-regulated during times of increased protein catabolism, as amino acid breakdown produces large amounts of NH4+. When amino acid catabolism increases, N-Acetylglutamate synthase is up-regulated, producing more N-acetyl-L-glutamate, which up-regulates carbamoyl phosphate synthetase and allows it to dispose of the excess NH4+ from catabolism.Aminoacylase is up-regulated during times of nutrient deficit or starvation, causing N-acetyl-L-glutamate breakdown, which down-regulates carbamoyl phosphate synthetase and the rest of the urea cycle. This response is evolutionarily advantageous, since a nutrient deficit means there isn't as much NH4+ that needs to be disposed of and since the body wants to salvage as many amino acids as it can.