Histone H3 (acetyl K4) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH03-71] (HA750905)
Catalog# HA750905
Rabbit Monoclonal to Acetyl-Histone H3 (K4)
Tested for
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WB
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IF-Cell
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ChIP
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Dot Blot
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Histone H3 (acetyl K4) on different lysates with Rabbit anti-Histone H3 (acetyl K4) antibody (HA750905) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 1 minute 22 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750905) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Histone H3 (acetyl K4) on different lysates with Rabbit anti-Histone H3 (acetyl K4) antibody (HA750905) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 1 minute 22 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750905) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.