Lane 1: Hela cell lysate, untreated
Lane 2: Hela cell lysate, treated with trichostatin at 500 ng/ml for 4h
Rabbit polyclonal primary
Acetyl-Histone H3 (K27) Rabbit Polyclonal Antibody (HA500046)
Synthetic peptide within human histone h3 (acetyl k27) aa 1-50.
Hela cell lysate, Hela cell lysate treated with trichostatin at 500 ng/ml for 4h, Hela cell lysate treated with Sodium Butyrate 0.5mM for 24h, mouse colon tissue, human colon tissue, human colon carcinoma tissue.
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Peptide affinity purified.
Acetyl-Histone H3 (K27)
Chromosome, Nucleosome core, Nucleus.
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human and mouse Histone H4 are subject to methylation at Lys 20, a modification that may be necessary for select DNA transactions or chromatin state transitions.