PRODUCT CODE: ET1602-34

Acetyl-Histone H2A (K9) Recombinant Rabbit Monoclonal Antibody [SR4-15] (ET1602-34)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Histone H2A(acetyl K9) on different cell lysates using anti-Histone H2A(acetyl K9) antibody at 1/500 dilution.<br />
Positive control:   <br />
Lane 1: HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates <br />
Lane 2: Untreated HeLa whole cell lysates
  • Western blot analysis of Histone H2A(acetyl K9) on different cell lysates using anti-Histone H2A(acetyl K9) antibody at 1/500 dilution.<br />
Positive control:   <br />
Lane 1: HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates <br />
Lane 2: Untreated HeLa whole cell lysates
  • ICC staining Histone H2A(acetyl K9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Histone H2A(acetyl K9) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Histone H2A(acetyl K9) in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.
  • mmunohistochemical analysis of paraffin-embedded human colon tissue using anti-Histone H2A(acetyl K9) antibody.The counter was dyed with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2A(acetyl K9) antibody.The counter was dyed with hematoxylin.
Western blot analysis of Histone H2A(acetyl K9) on different cell lysates using anti-Histone H2A(acetyl K9) antibody at 1/500 dilution.
Positive control:
Lane 1: HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates
Lane 2: Untreated HeLa whole cell lysates

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Acetyl-Histone H2A (K9) Recombinant Rabbit Monoclonal Antibody [SR4-15] (ET1602-34)

Immunogen

Recombinant protein

Host

Rabbit

Modification

Acetyl

Modification Site

K9

Positive Control

A549, MCF-7, Hela, human breast carcinoma tissue, human tonsil tissue, mouse testis tissue, human lung carcinoma tissue, mouse colon tissue, human colon cancer tissue, mouse thyroid tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SR4-15

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

14 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Acetyl-Histone H2A (K9)

SYNONYMS

H2A antibody; H2A1B_HUMAN antibody; H2AFM antibody; HIST1H2A antibody; HIST1H2AE antibody; Histone H2A type 1-B/E antibody; Histone H2A.2 antibody; Histone H2A/a antibody; Histone H2A/m antibody

SEQUENCE SIMILARITIES

Belongs to the histone H2A family.

POST-TRANSLATIONAL MODIFICATION

Deiminated on Arg-4 in granulocytes upon calcium entry.; Monoubiquitination of Lys-120 (H2AK119Ub) by RING1, TRIM37 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3 (H3K27me). H2AK119Ub by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Monoubiquitination of Lys-120 (H2AK119Ub) by TRIM37 may promote transformation of cells in a number of breast cancers. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Ubiquitination at Lys-14 and Lys-16 (H2AK13Ub and H2AK15Ub, respectively) in response to DNA damage is initiated by RNF168 that mediates monoubiquitination at these 2 sites, and 'Lys-63'-linked ubiquitin are then conjugated to monoubiquitin; RNF8 is able to extend 'Lys-63'-linked ubiquitin chains in vitro. Deubiquitinated by USP51 at Lys-14 and Lys-16 (H2AK13Ub and H2AK15Ub, respectively) after damaged DNA is repaired. H2AK119Ub and ionizing radiation-induced 'Lys-63'-linked ubiquitination (H2AK13Ub and H2AK15Ub) are distinct events.; Phosphorylation on Ser-2 (H2AS1ph) is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1. Phosphorylation at Thr-121 (H2AT120ph) by DCAF1 is present in the regulatory region of many tumor suppresor genes and down-regulates their transcription.; Glutamine methylation at Gln-105 (H2AQ104me) by FBL is specifically dedicated to polymerase I. It is present at 35S ribosomal DNA locus and impairs binding of the FACT complex.; Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.; Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.

SUBCELLULAR LOCATION

Nucleus, Chromosome.

FUNCTION

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

CITATIONS

  • Qin, Ge et al.

    Panobinostat (LBH589) inhibits Wnt/β-catenin signaling pathway via upregulating APCL expression in breast cancer. | Cellular Signalling [2019]