alpha Tubulin (acetyl K40) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH19-53] (HA751717)
Catalog# HA751717
Rabbit Monoclonal to Acetyl-alpha Tubulin (K40)
Tested for
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WB
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IP
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IF-Cell
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of alpha Tubulin (acetyl K40) on different lysates with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA751717) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate (20 µg/Lane)
Lane 5: C6 cell lysate
Lane 6: C6 treated with 1μM TSA for 18 hours cell lysate (20 µg/Lane)
Lane 7: Mouse brain tissue lysate (40 µg/Lane)
Lane 8: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: Lane 1-6: 6 seconds; Lane 7-8: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751717) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of alpha Tubulin (acetyl K40) on different lysates with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA751717) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate (20 µg/Lane)
Lane 5: C6 cell lysate
Lane 6: C6 treated with 1μM TSA for 18 hours cell lysate (20 µg/Lane)
Lane 7: Mouse brain tissue lysate (40 µg/Lane)
Lane 8: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: Lane 1-6: 6 seconds; Lane 7-8: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751717) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.