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PRODUCT CODE: ER1902-40

ACE2 Rabbit Polyclonal Antibody (ER1902-40)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of ACE2 on human kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of ACE2 on human kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-40, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of ACE2 was done on Vero cells. The cells were stained with the primary antibody (ER1902-40, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; green).
  • Flow cytometric analysis of ACE2 was done with ACE2 transfected 293 cells. The cells were stained with the primary antibody (ER1902-40, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; green).
Western blot analysis of ACE2 on human kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ACE2 Rabbit Polyclonal Antibody (ER1902-40)

Immunogen

Ace2 transfected 293 cells.

Host

Rabbit

Positive Control

Human kidney tissue lysates, human kidney tissue, Vero cell, ACE2 transfected 293 cells.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

Predicted band size: 64/92 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P:1:50-1:200

  • FC

  • 1:50

TARGET

UNIPROT #

Q9BYF1

PROTEIN NAME

ACE2

SYNONYMS

ACE 2 antibody ; ACE related carboxypeptidase antibody ; ACE-related carboxypeptidase antibody ; ACE2 antibody ; ACE2_HUMAN antibody ; ACEH antibody ; Angiotensin converting enzyme 2 antibody ; Angiotensin converting enzyme homolog antibody ; Angiotensin converting enzyme like protein antibody ; Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody ; Angiotensin I converting enzyme 2 antibody ; Angiotensin-converting enzyme homolog antibody ; DKFZP434A014 antibody ; EC 3.4.17 antibody ; metalloprotease MPROT 15 antibody ; Metalloprotease MPROT15 antibody ; OTTHUMP00000022963 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase M2 family.

TISSUE SPECIFICITY

Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells (at protein level). Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in the renal proximal tubule and the small intestine (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.

POST-TRANSLATIONAL MODIFICATION

N-glycosylation on Asn-90 may limit SARS infectivity.; Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1.

SUBCELLULAR LOCATION

Secreted, Cell membrane.

FUNCTION

Angiotensin-converting enzyme (ACE) is a carboxyl-terminal dipeptidyl exopeptidase that converts angiotensin I to the potent vasopressive hormone, angiotensin II. There are two isoforms of ACE, the pulmonary ACEP and the testicular ACET. ACEP is a glycoprotein expressed in vascular endothelial cells of the lung, liver, adrenal cortex, pancreas, kidney and spleen. The ACET isoform is expressed exclusively in adult testis by developing sperm cells, specifically late pachytene spermatocytes. Additionally, ACE inactivates bradykinin, a vasodepressor peptide, and is involved in blood pressure regulation and fluid/electrolyte homeostasis. ACE2 is the first known human homolog of ACE. Unlike ACE, which is expressed ubiquitously throughout the vasculature, ACE2 is expressed only in cardiac, renal and testicular cells. ACE2 is not sensitive to the ACE inhibitor drugs used to treat hypertension. ACE2 receptors have been shown to be the entry point into human cells for some coronaviruses, including the SARS virus[10]. A number of studies have identified that the entry point is the same for SARS-CoV-2, the COVID-19 virus.