ACAT-1 Recombinant Rabbit Monoclonal Antibody [JE64-40] (HA721093)
Catalog# HA721093
Rabbit Monoclonal to ACAT1
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
Western blot analysis of ACAT-1 on different lysates with Rabbit anti-ACAT-1 antibody (HA721093) at 1/2,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si ACAT-1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 42 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721093) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ACAT-1 Recombinant Rabbit Monoclonal Antibody [JE64-40] (HA721093)
Immunogen
Synthetic peptide within human acat1 aa 378-427/427.
Host
Rabbit
Positive Control
Raji cell lysate, HepG2 cell lysate, rat liver tissue lysate, rat heart tissue lysate, HepG2, human liver tissue, human liver carcinoma tissue, rat liver tissue, mouse liver tissue, mouse heart tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE64-40
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IHC-P
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1:100-1:400
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IF-Cell
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1:250
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 45 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ACAT1
SYNONYMS
ACAT 1 antibody;ACAT antibody;acat1 antibody;Acetoacetyl CoA thiolase antibody;acetoacetyl Coenzyme A thiolase antibody;Acetoacetyl-CoA thiolase antibody;Acetyl CoA acetyltransferase, mitochondrial antibody;Acetyl Coenzyme A acetyltransferase 1 antibody;Acetyl-CoA acetyltransferase antibody;acetyl-coa acetyltransferase precursor, mitochondrial antibody;Acetyl-CoA thiolase, mitochondrial antibody;acetyl-Coenzyme A acetyltransferase 1 antibody;MAT antibody;mitochondrial acetoacetyl-CoA thiolase antibody;mitochondrial antibody;RATACAL antibody;T2 antibody;testicular tissue protein Li 198 antibody;THIL antibody;THIL_HUMAN antibody
SUBCELLULAR LOCATION
Mitochondrion.
FUNCTION
Acetyl-CoA acetyltransferase, mitochondrial, also known as acetoacetyl-CoA thiolase, is an enzyme that in humans is encoded by the ACAT1 (Acetyl-Coenzyme A acetyltransferase 1) gene. Acetyl-Coenzyme A acetyltransferase 1 is an acetyl-CoA C-acetyltransferase enzyme. This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. The ACAT1 enzyme has a few unique properties. First, it is activated by potassium ions binding near the CoA binding site and the catalytic site. This binding causes a structural change in the active site loop. Additionally, this enzyme is able to use 2-methyl-branched acetoacetyl-CoA as a substrate, making it a unique thiolase. ACAT1 is regulated at both transcriptional and translational levels. ACAT1 enzyme activity is enhanced ACAT1’s expression is promoted transcriptionally by leptin, angiotensin II, and insulin in human monocytes/macrophages. Insulin-mediated regulation also involves ERK, p38MAPK, and JNK signaling pathways.
CITATIONS
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Acetyl-coenzyme A acetyltransferase 1 promotes brown adipogenesis by activating the AMPK-PGC1α signaling pathway
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WB
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Mouse
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Citation
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