PRODUCT CODE: HA720006

ACAA2 Recombinant Rabbit Monoclonal Antibody [JE56-67] (HA720006)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of ACAA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720006, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: 293T cell lysate<br />
Lane 2: Rat colon tissue lysate<br />
Lane 3: NIH/3T3 cell lysate
  • Western blot analysis of ACAA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720006, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: 293T cell lysate<br />
Lane 2: Rat colon tissue lysate<br />
Lane 3: NIH/3T3 cell lysate
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ACAA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720006, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ACAA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720006, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-ACAA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720006, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of ACAA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720006, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293T cell lysate
Lane 2: Rat colon tissue lysate
Lane 3: NIH/3T3 cell lysate

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

ACAA2 Recombinant Rabbit Monoclonal Antibody [JE56-67] (HA720006)

Immunogen

Synthetic peptide within c-terminal human acaa2.

Host

Rabbit

Positive Control

293T cell lysate, rat colon tissue lysate, NIH/3T3 cell lysate, rat kidney tissue, human liver tissue, human appendix tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE56-67

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

42 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

ACAA2

SYNONYMS

3 ketoacyl CoA thiolase antibody; 3 ketoacyl CoA thiolase mitochondrial antibody; 3-ketoacyl-CoA thiolase, mitochondrial antibody; 3-oxoacyl-CoA thiolase, mitochondrial antibody; ACAA 2 antibody; Acaa2 antibody; Acetyl CoA acyltransferase 2 antibody; Acetyl CoA acyltransferase antibody; Acetyl Coenzyme A acyltransferase 2 antibody; Acetyl-CoA acyltransferase antibody; Beta ketothiolase antibody; Beta-ketothiolase antibody; DSAEC antibody; Mitochondrial 3 oxoacyl CoA thiolase antibody; Mitochondrial 3 oxoacyl Coenzyme A thiolase antibody; Mitochondrial 3-oxoacyl-CoA thiolase antibody; T1 antibody; THIM_HUMAN antibody

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

ACAA2 Antibody (192) is a high quality monoclonal ACAA2 antibody (also designated ACAA2 antibody) suitable for the detection of the ACAA2 protein of mouse, rat and human origin. ACAA2 Antibody (192) is available as the non-conjugated anti-ACAA2 antibody. Mammalian tissues contain five types of thiolases, all of which participate in metabolism of various compounds throughout the body. ACAA2 (acetyl-Coenzyme A acyltransferase 2), also known as DSAEC, is a 397 amino acid member of the thiolase family of enzymes and is involved in lipid metabolism. Localized to the mitochondrion, ACAA2 catalyzes the last step, namely the conversion of acetyl-CoA to 3-oxoacyl-CoA, in the fatty acid oxidation pathway. ACAA2 is highly expressed in liver, fibrofblasts and intercostal muscle and contains an N-terminal targeting signal that, unlike other mitochondrial proteins, is non-cleavable. Human ACAA2 shares 86.6% amino acid identity with its rat counterpart, suggesting a conserved function for ACAA2 among different species.