PRODUCT CODE: ET1702-40

ABCG2 Recombinant Rabbit Monoclonal Antibody [JF0994] (ET1702-40)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of ABCG2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: HepG2 cell lysate<br />
Lane 2: human placenta tissue lysate<br />
Lane 3: Hela cell lysate<br />
Lane 4: 293T cell lysate
  • Western blot analysis of ABCG2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: HepG2 cell lysate<br />
Lane 2: human placenta tissue lysate<br />
Lane 3: Hela cell lysate<br />
Lane 4: 293T cell lysate
  • ICC staining of ABCG2 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of ABCG2 in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of ABCG2 in 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ABCG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of ABCG2 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-40, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of ABCG2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: human placenta tissue lysate
Lane 3: Hela cell lysate
Lane 4: 293T cell lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

ABCG2 Recombinant Rabbit Monoclonal Antibody [JF0994] (ET1702-40)

Immunogen

Synthetic peptide within human abcg2 aa 140-180.

Host

Rabbit

Positive Control

HepG2 cell lysate, human placenta tissue lysate, Hela cell lysate, 293T cell lysate, Hela, MCF-7, 293T, mouse placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0994

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

72 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

ABCG2

SYNONYMS

ABC transporter antibody; ABC15 antibody; ABCG 2 antibody; ABCG2 antibody; ABCG2_HUMAN antibody; ABCP antibody; ATP binding cassette sub family G (WHITE) member 2 antibody; ATP binding cassette transporter G2 antibody; ATP-binding cassette sub-family G member 2 antibody; BCRP antibody; BCRP1 antibody; BMDP antibody; Breast cancer resistance protein antibody; CD338 antibody; CDw338 antibody; CDw338 antigen antibody; EST157481 antibody; GOUT1 antibody; MGC102821 antibody; Mitoxantrone resistance associated protein antibody; Mitoxantrone resistance-associated protein antibody; MRX antibody; Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody; MXR antibody; MXR1 antibody; Placenta specific ATP binding cassette transporter antibody; Placenta specific MDR protein antibody; Placenta-specific ATP-binding cassette transporter antibody; UAQTL1 antibody

SEQUENCE SIMILARITIES

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

TISSUE SPECIFICITY

Highly expressed in placenta. Low expression in small intestine, liver and colon. Expressed in brain (at protein level).

POST-TRANSLATIONAL MODIFICATION

N-glycosylated. Glycosylation-deficient ABCG2 is normally expressed and functional.; Phosphorylated. Phosphorylation at Thr-362 by PIM1 is induced by drugs like mitoxantrone and is associated with cells increased drug resistance. It regulates the localization to the plasma membrane, the homooligomerization and therefore, the activity of the transporter.

SUBCELLULAR LOCATION

Mitochondrion membrane, Cell membrane, Apical cell membrane.

FUNCTION

ATP-binding cassette (ABC) transporters are an evolutionarily conserved family of proteins that catalyze the transport of molecules across extracellular and intracellular membranes through the energy of ATP hydrolysis. The ABC half-transporter, ABCG2, is also known as placenta-specific ABC transporter and breast cancer resistance protein (BCRP1). ABCG2 confers resistance for a variety of chemotherapeutic agents, including anthracyclines, mitoxantrone, bisantrene and topotecan. Under normal conditions, ABCG2 may serve a protective function by removing toxins from the cell, and plays an important role in regulating stem cell differentiation. ABCG2 is responsible for the side population (SP) phenotype and is widely expressed in a large variety of stem cells, making it an important stem cell marker. ABCG2 may have N-linked glycosylation and may dimerize in vivo. ABCG2 is abundantly expressed in placenta, liver, intestine and stem cells.