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PRODUCT CODE: ET1608-20

ABCG1 Recombinant Rabbit Monoclonal Antibody [SU03-26] (ET1608-20)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Rat

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Western blot analysis of ABCG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: THP-1 cell lysate<br /> Lane 2: MCF-7 cell lysate
  • Western blot analysis of ABCG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: THP-1 cell lysate<br /> Lane 2: MCF-7 cell lysate
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-ABCG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-ABCG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of ABCG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: THP-1 cell lysate
Lane 2: MCF-7 cell lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

ABCG1 Recombinant Rabbit Monoclonal Antibody [SU03-26] (ET1608-20)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

THP-1 cell lysate, MCF-7 cell lysate, human lung tissue, human skin tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU03-26

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

110 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

P45844

PROTEIN NAME

ABCG1

SYNONYMS

ABC transporter 8 antibody; ABC8 antibody; ABCG1 antibody; ABCG1_HUMAN antibody; ATP-binding cassette sub family G member 1 antibody; ATP-binding cassette sub-family G member 1 antibody; ATP-binding cassette transporter 8 antibody; ATP-binding cassette transporter member 1 of subfamily G antibody; ATP-binding cassette, sub family G WHITE member 1 antibody; homolog of Drosophila white antibody; MGC34313 antibody; White protein homolog antibody; White protein homolog ATP binding cassette transporter 8 antibody; WHITE1 antibody; WHT1 antibody

SEQUENCE SIMILARITIES

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

TISSUE SPECIFICITY

Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.

POST-TRANSLATIONAL MODIFICATION

Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane, Golgi apparatus membrane.

FUNCTION

ABCG1 (also designated ABC8 or human white gene), a member of the evolutionary conserved family of ATP-binding cassette (ABC) transporters, exhibits high homology with the Drosophila white gene. ABC transporters couple the energy of ATP hydrolysis to the translocation of various molecules across biological membranes. These proteins contain characteristic ATP-binding domains and transmembrane domains which form a channel-like structure for transport. ABCG1 functions to regulate cholesterol and phospholipid transport in macrophages. ABCG1 is highly expressed in several tissues, including brain, spleen, lung and placenta, and has been localized to the cell surface and intracellular compartments of cholesterol-laden macrophages.