Positive control:
Lane 1: THP-1 cell lysate
Lane 2: MCF-7 cell lysate
Applications
-
WB
-
ICC
-
IF
-
IHC-P
REACTIVITY
-
Human
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ABCG1 Recombinant Rabbit Monoclonal Antibody [SU03-26] (ET1608-20)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
THP-1 cell lysate, MCF-7 cell lysate, human lung tissue, human skin tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU03-26
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
110 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
ABCG1
SYNONYMS
ABC transporter 8 antibody; ABC8 antibody; ABCG1 antibody; ABCG1_HUMAN antibody; ATP-binding cassette sub family G member 1 antibody; ATP-binding cassette sub-family G member 1 antibody; ATP-binding cassette transporter 8 antibody; ATP-binding cassette transporter member 1 of subfamily G antibody; ATP-binding cassette, sub family G WHITE member 1 antibody; homolog of Drosophila white antibody; MGC34313 antibody; White protein homolog antibody; White protein homolog ATP binding cassette transporter 8 antibody; WHITE1 antibody; WHT1 antibody
SEQUENCE SIMILARITIES
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
TISSUE SPECIFICITY
Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
POST-TRANSLATIONAL MODIFICATION
Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum.
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane, Golgi apparatus membrane.
FUNCTION
ABCG1 (also designated ABC8 or human white gene), a member of the evolutionary conserved family of ATP-binding cassette (ABC) transporters, exhibits high homology with the Drosophila white gene. ABC transporters couple the energy of ATP hydrolysis to the translocation of various molecules across biological membranes. These proteins contain characteristic ATP-binding domains and transmembrane domains which form a channel-like structure for transport. ABCG1 functions to regulate cholesterol and phospholipid transport in macrophages. ABCG1 is highly expressed in several tissues, including brain, spleen, lung and placenta, and has been localized to the cell surface and intracellular compartments of cholesterol-laden macrophages.