PRODUCT CODE: ET7110-01

ABAT/GABA-T Recombinant Rabbit Monoclonal Antibody [JE50-16] (ET7110-01)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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+
Western blot analysis of ABAT/GABA-T on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-01, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Human liver tissue lysate<br />
Lane 2: Rat liver tissue lysate
  • Western blot analysis of ABAT/GABA-T on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-01, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Human liver tissue lysate<br />
Lane 2: Rat liver tissue lysate
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ABAT/GABA-T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-01, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ABAT/GABA-T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-01, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ABAT/GABA-T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-01, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of ABAT/GABA-T on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-01, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human liver tissue lysate
Lane 2: Rat liver tissue lysate

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

ABAT/GABA-T Recombinant Rabbit Monoclonal Antibody [JE50-16] (ET7110-01)

Immunogen

Synthetic peptide within n-terminal human abat/gaba-t.

Host

Rabbit

Positive Control

Human liver tissue, rat liver tissue, human liver cancer tissue, human placenta tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE50-16

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

52 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:1,000

  • IP:1:50 IHC-P:1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

ABAT/GABA-T

SYNONYMS

(S) 3 amino 2 methylpropionate transaminase antibody; (S)-3-amino-2-methylpropionate transaminase antibody; 4 aminobutyrate aminotransferase antibody; 4 aminobutyrate aminotransferase, mitochondrial antibody; 4-aminobutyrate aminotransferase antibody; ABAT antibody; FLJ17813 antibody; FLJ30272 antibody; GABA aminotransferase antibody; GABA AT antibody; GABA T antibody; GABA transaminase antibody; GABA transferase antibody; GABA-AT antibody; GABA-T antibody; GABAT antibody; GABT_HUMAN antibody; Gamma amino N butyrate transaminase antibody; Gamma-amino-N-butyrate transaminase antibody; hCG1984265 antibody; L AIBAT antibody; L-AIBAT antibody; LAIBAT antibody; mitochondrial antibody; NPD009 antibody

SEQUENCE SIMILARITIES

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

TISSUE SPECIFICITY

Liver > pancreas > brain > kidney > heart > placenta.

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.