UGP2 Rabbit Polyclonal Antibody
Catalog# R1404-5
UGP2 Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
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Rat
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Zebrafish
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unconjugated
Overview
Product Name
UGP2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human UGP2 aa 60-120.
Species Reactivity
Human, Mouse, Rat, Zebrafish
Validated Applications
WB
Molecular Weight
Predicted band size: 57 kDa
Positive Control
Human liver, rat liver, mouse liver, human heart, NIH/3T3, HepG2
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
Target
Function
UTP—glucose-1-phosphate uridylyltransferase also known as glucose-1-phosphate uridylyltransferase (or UDP–glucose pyrophosphorylase) is an enzyme associated with glycogenesis. It synthesizes UDP-glucose from glucose-1-phosphate and UTP. It plays a central role as a glucosyl donor in cellular metabolic pathways.
Background References
1. "The importance of conserved residues in human liver UDPglucose pyrophosphorylase." Chang H.-Y., Peng H.-L., Chao Y.C., Duggleby R.G. Eur. J. Biochem. 236:723-728(1996)
2. "The crystal structure of human UDP-glucose pyrophosphorylase reveals a latch effect that influences enzymatic activity." Yu Q., Zheng X. Biochem. J. 442:283-291(2012)
Sequence Similarity
Belongs to the UDPGP type 1 family.
Subcellular Location
Cytoplasm
UNIPROT
Synonyms
pHC379 antibody
UDP glucose diphosphorylase antibody
UDP glucose pyrophosphorylase 1 antibody
UDP glucose pyrophosphorylase 2 antibody
UDP glucose pyrophosphorylase antibody
UDP-glucose pyrophosphorylase antibody
UDPG antibody
UDPGP 2 antibody
UDPGP antibody
UDPGP2 antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of UGP2 on different lysates with Rabbit anti-UGP2 antibody (R1404-5) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-UGP2 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 57/56 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1404-5) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis on cell lysates using anti- UGP2 rabbit polyclonal antibodies.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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