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UGP2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1404-5

UGP2 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

  • Zebrafish

Conjugation

  • unconjugated

Overview

Product Name

UGP2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human UGP2 aa 60-120.

Species Reactivity

Human, Mouse, Rat, Zebrafish

Validated Applications

WB

Target Molecular Weight

Predicted band size: 57 kDa

Positive Control

Human liver tissue lysate, Rat liver tissue lysate, Mouse liver tissue lysate

Conjugation

unconjugated

RRID

AB_3073498

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

UTP—glucose-1-phosphate uridylyltransferase also known as glucose-1-phosphate uridylyltransferase (or UDP–glucose pyrophosphorylase) is an enzyme associated with glycogenesis. It synthesizes UDP-glucose from glucose-1-phosphate and UTP. It plays a central role as a glucosyl donor in cellular metabolic pathways.

Background References

1. "The importance of conserved residues in human liver UDPglucose pyrophosphorylase." Chang H.-Y., Peng H.-L., Chao Y.C., Duggleby R.G. Eur. J. Biochem. 236:723-728(1996)

2. "The crystal structure of human UDP-glucose pyrophosphorylase reveals a latch effect that influences enzymatic activity." Yu Q., Zheng X. Biochem. J. 442:283-291(2012)

Sequence Similarity

Belongs to the UDPGP type 1 family.

Subcellular Location

Cytoplasm

UNIPROT

SwissProt: Q16851 Human

Synonyms

pHC379 antibody

UDP glucose diphosphorylase antibody

UDP glucose pyrophosphorylase 1 antibody

UDP glucose pyrophosphorylase 2 antibody

UDP glucose pyrophosphorylase antibody

UDP-glucose pyrophosphorylase antibody

UDPG antibody

UDPGP 2 antibody

UDPGP antibody

UDPGP2 antibody

Expand

Images

  • Western blot analysis of UGP2 on different lysates with Rabbit anti-UGP2 antibody (<a href="/products/R1404-5" style="font-weight: bold;text-decoration: underline;">R1404-5</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human liver tissue lysate<br />Lane 2: Rat liver tissue lysate<br />Lane 3: Mouse liver tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/R1404-5" style="font-weight: bold;text-decoration: underline;">R1404-5</a>, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature

    Western blot analysis of UGP2 on different lysates with Rabbit anti-UGP2 antibody (R1404-5) at 1/1,000 dilution.

    Lane 1: Human liver tissue lysate
    Lane 2: Rat liver tissue lysate
    Lane 3: Mouse liver tissue lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: R1404-5, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of UGP2 on different lysates with Rabbit anti-UGP2 antibody (<a href="/products/R1404-5" style="font-weight: bold;text-decoration: underline;">R1404-5</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-UGP2 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 57 kDa<br />Observed band size: 57/56 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1404-5" style="font-weight: bold;text-decoration: underline;">R1404-5</a>) at 1/1,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of UGP2 on different lysates with Rabbit anti-UGP2 antibody (R1404-5) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-UGP2 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 57 kDa
    Observed band size: 57/56 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1404-5) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Alternative Products

metafield image

UGP2 Mouse Monoclonal Antibody [7H1]

Application: WB,IHC-P,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated