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SIX2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER2001-39

SIX2 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

SIX2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within mouse six2 aa 100-160.

Species Reactivity

Mouse

Validated Applications

WB

Molecular Weight

32 kDa

Positive Control

Mouse kidney tissue lysate, mouse stomach tissue lysate.

Conjugation

unconjugated

RRID

AB_3069516

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500

Target

Function

Transcription factor that plays an important role in the development of several organs, including kidney, skull and stomach. During kidney development, maintains cap mesenchyme multipotent nephron progenitor cells in an undifferentiated state by opposing the inductive signals emanating from the ureteric bud and cooperates with WNT9B to promote renewing progenitor cells proliferation. Acts through its interaction with TCF7L2 and OSR1 in a canonical Wnt signaling independent manner preventing transcription of differentiation genes in cap mesenchyme such as WNT4. Also acts independently of OSR1 to activate expression of many cap mesenchyme genes, including itself, GDNF and OSR1. During craniofacial development plays a role in growth and elongation of the cranial base through regulation of chondrocyte differentiation . During stomach organogenesis, controls pyloric sphincter formation and mucosal growth through regulation of a gene network including NKX2-5, BMPR1B, BMP4, SOX9 and GREM1 . During branchial arch development, acts to mediate HOXA2 control over the insulin-like growth factor pathway . Also may be involved in limb tendon and ligament development. Plays a role in cell proliferation and migration (By similarity).

Background References

1. Oliver G. et. al. Homeobox genes and connective tissue patterning. Development 121:693-705(1995).

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q62232 Mouse

Synonyms

Homeobox protein SIX2 antibody

Sine oculis homeobox homolog 2 antibody

Six2 antibody

six2a antibody

Images

  • Western blot analysis of SIX2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-39" style="font-weight: bold;text-decoration: underline;">ER2001-39</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: Mouse kidney tissue lysate<br />Lane 2: Mouse stomach tissue lysate

    Western blot analysis of SIX2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse kidney tissue lysate
    Lane 2: Mouse stomach tissue lysate

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"