SIX2 Rabbit Polyclonal Antibody
Catalog# ER2001-39
SIX2 Rabbit Polyclonal Antibody
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WB
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Mouse
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unconjugated
Overview
Product Name
SIX2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within mouse six2 aa 100-160.
Species Reactivity
Mouse
Validated Applications
WB
Target Molecular Weight
32 kDa
Positive Control
Mouse kidney tissue lysate, mouse stomach tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1mg/ml.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:500
Target
Function
Transcription factor that plays an important role in the development of several organs, including kidney, skull and stomach. During kidney development, maintains cap mesenchyme multipotent nephron progenitor cells in an undifferentiated state by opposing the inductive signals emanating from the ureteric bud and cooperates with WNT9B to promote renewing progenitor cells proliferation. Acts through its interaction with TCF7L2 and OSR1 in a canonical Wnt signaling independent manner preventing transcription of differentiation genes in cap mesenchyme such as WNT4. Also acts independently of OSR1 to activate expression of many cap mesenchyme genes, including itself, GDNF and OSR1. During craniofacial development plays a role in growth and elongation of the cranial base through regulation of chondrocyte differentiation . During stomach organogenesis, controls pyloric sphincter formation and mucosal growth through regulation of a gene network including NKX2-5, BMPR1B, BMP4, SOX9 and GREM1 . During branchial arch development, acts to mediate HOXA2 control over the insulin-like growth factor pathway . Also may be involved in limb tendon and ligament development. Plays a role in cell proliferation and migration (By similarity).
Background References
1. Oliver G. et. al. Homeobox genes and connective tissue patterning. Development 121:693-705(1995).
Subcellular Location
Nucleus.
UNIPROT
Synonyms
Homeobox protein SIX2 antibody
Sine oculis homeobox homolog 2 antibody
Six2 antibody
six2a antibody
Images
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Western blot analysis of SIX2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse kidney tissue lysate
Lane 2: Mouse stomach tissue lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"