5HT2C Receptor Recombinant Rabbit Monoclonal Antibody [JA31-31]
Catalog# ET1704-87
5HT2C Receptor Recombinant Rabbit Monoclonal Antibody [JA31-31]
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WB
-
IHC-P
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Human
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Mouse
-
Rat
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unconjugated
Overview
Product Name
5HT2C Receptor Recombinant Rabbit Monoclonal Antibody [JA31-31]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human 5HT2C Receptor aa 409-458 / 458.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 52 kDa
Positive Control
K-562 cell lysate, SH-SY5Y cell lysate, human brain tissue lysate, mouse brain tissue, rat brain tissue.
Conjugation
unconjugated
Clone Number
JA31-31
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:50
Target
Function
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone.
Background References
1. Jahnsen JA1, Uhlén S. The N-terminal region of the human 5-HT C receptor has as a cleavable signal peptide. Eur J Pharmacol 684(1-3):44-50 (2012).
2. Knauer CS et al. Pharmacological characterization of mitogen-activated protein kinase activation by recombinant human 5-HT2C, 5-HT2A, and 5-HT2B receptors. Naunyn Schmiedebergs Arch Pharmacol 379(5):461-71 (2009).
Sequence Similarity
Belongs to the G-protein coupled receptor 1 family.
Tissue Specificity
Detected in brain.
Post-translational Modification
N-glycosylated.
Subcellular Location
Cell membrane.
Synonyms
5 Hydroxytryptamine 2C receptor antibody
5-HT-1C antibody
5-ht-1c receptor antibody
5-HT-2C antibody
5-HT1C antibody
5-HT2C antibody
5-HTR2C antibody
5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled antibody
5-hydroxytryptamine receptor 1C antibody
5-hydroxytryptamine receptor 2C antibody
ExpandImages
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Western blot analysis of 5HT2C Receptor on different lysates with Rabbit anti-5HT2C Receptor antibody (ET1704-87) at 1/1,000 dilution.
Lane 1: K-562 cell lysate (15 µg/Lane)
Lane 2: SH-SY5Y cell lysate (15 µg/Lane)
Lane 3: Human brain tissue lysate (30 µg/Lane)
Predicted band size: 52 kDa
Observed band size: 70 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-87) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-5HT2C Receptor antibody (ET1704-87) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-87) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-5HT2C Receptor antibody (ET1704-87) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-87) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"