5HT2C Receptor Recombinant Rabbit Monoclonal Antibody [JA31-31]
Catalog# ET1704-87
5HT2C Receptor Recombinant Rabbit Monoclonal Antibody [JA31-31]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
5HT2C Receptor Recombinant Rabbit Monoclonal Antibody [JA31-31]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human 5HT2C Receptor aa 409-458 / 458.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 52 kDa
Positive Control
K-562 cell lysate, SH-SY5Y cell lysate, human brain tissue lysate, mouse brain tissue, rat brain tissue.
Conjugation
unconjugated
Clone Number
JA31-31
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:500-1:2,000
Target
Function
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone.
Background References
1. Jahnsen JA1, Uhlén S. The N-terminal region of the human 5-HT C receptor has as a cleavable signal peptide. Eur J Pharmacol 684(1-3):44-50 (2012).
2. Knauer CS et al. Pharmacological characterization of mitogen-activated protein kinase activation by recombinant human 5-HT2C, 5-HT2A, and 5-HT2B receptors. Naunyn Schmiedebergs Arch Pharmacol 379(5):461-71 (2009).
Sequence Similarity
Belongs to the G-protein coupled receptor 1 family.
Tissue Specificity
Detected in brain.
Post-translational Modification
N-glycosylated.
Subcellular Location
Cell membrane.
Synonyms
5 Hydroxytryptamine 2C receptor antibody
5-HT-1C antibody
5-ht-1c receptor antibody
5-HT-2C antibody
5-HT1C antibody
5-HT2C antibody
5-HTR2C antibody
5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled antibody
5-hydroxytryptamine receptor 1C antibody
5-hydroxytryptamine receptor 2C antibody
ExpandImages
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Western blot analysis of 5HT2C Receptor on different lysates with Rabbit anti-5HT2C Receptor antibody (ET1704-87) at 1/1,000 dilution.
Lane 1: K-562 cell lysate (15 µg/Lane)
Lane 2: SH-SY5Y cell lysate (15 µg/Lane)
Lane 3: Human brain tissue lysate (30 µg/Lane)
Predicted band size: 52 kDa
Observed band size: 70 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-87) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"