RAB27B Rabbit Polyclonal Antibody
Catalog# ER2001-10
RAB27B Rabbit Polyclonal Antibody
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WB
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FC
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Human
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unconjugated
Overview
Product Name
RAB27B Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide with N-terminal Human RAB27B.
Species Reactivity
Human
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 25 kDa
Positive Control
U937 cell lysates, SH-SY5Y.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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FC
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1:500-1:1,000
Target
Function
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion ref. Plays a role in NTRK2/TRKB axonal anterograde transport by facilitating the association of NTRK2/TRKB with KLC1 ref. May be involved in targeting uroplakins to urothelial apical membranes (By similarity).
Background References
1. Zhang L. et. al. Rab27b Is a Potential Indicator for Lymph Node Metastasis and Unfavorable Prognosis in Lung Adenocarcinoma. Dis Markers. 2018 Dec
2. Gallo LI. et. al. RAB27B requirement for stretch-induced exocytosis in bladder umbrella cells. Am J Physiol Cell Physiol. 2018 Mar
Subcellular Location
Late endosome, Membrane, Lipid-anchor.
UNIPROT
Synonyms
C25KG antibody
Rab27b antibody
RAB27B member RAS oncogene family antibody
RAS associated protein RAB27B antibody
Ras related protein Rab 27b antibody
Ras related protein Rab27b antibody
Ras-related protein Rab-27B antibody
RB27B_HUMAN antibody
Small GTP binding protein rab27b antibody
Images
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Western blot analysis of RAB27B on U937 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Flow cytometric analysis of RAB27B was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-10, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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