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UBE1C Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1706-14

UBE1C Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

UBE1C Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Human UBE1C aa 220-424 / 463.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size: 52 kDa

Positive Control

Human liver tissue lysate, A431, HUVEC, SH-SY5Y, rat brain tissue, human pancreas tissue, mouse testis tissue.

Conjugation

unconjugated

RRID

AB_3073423

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Ubiquitin is an abundant, highly conserved protein found in all eukaryotic cells either free or covalently attached to cellular proteins. The primary function of ubiquitin in mammalian systems is to clear abnormal, foreign and improperly folded proteins by targeting them for proteosome degradation. UBA3 (ubiquitin-like modifier activating enzyme 3), also known as NEDD8-activating enzyme E1 catalytic subunit or UBE1C (ubiquitin-activating enzyme E1C), is a 463 amino acid protein belonging to the ubiquitin-activating E1 family and UBA3 subfamily. Ubiquitously expressed, UBA3 acts as an activator to NEDD8, a ubiquitin-like protein, thus regulating cell division, signaling and embryogenesis. UBA3 exists as two isoforms due to alternative splicing events.

Background References

1. Coleman KE et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Elife 6:N/A (2017).

2. Xu J et al. The neddylation-cullin 2-RBX1 E3 ligase axis targets tumor suppressor RhoB for degradation in liver cancer. Mol Cell Proteomics 14:499-509 (2015).

Sequence Similarity

Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.

Tissue Specificity

Ubiquitously expressed.

Subcellular Location

Nucleus. Cytoplasm.

UNIPROT

SwissProt: Q8TBC4 Human

SwissProt: Q8C878 Mouse

SwissProt: Q99MI7 Rat

Synonyms

DKFZp566J164 antibody

EC 6.3.2. antibody

hUba3 antibody

MGC22384 antibody

NEDD8 activating enzyme E1 catalytic subunit antibody

NEDD8 activating enzyme E1C antibody

Nedd8 activating enzyme hUba3 antibody

NEDD8-activating enzyme E1 catalytic subunit antibody

NEDD8-activating enzyme E1C antibody

uba3 antibody

Expand

Images

  • ICC staining UBE1C in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining UBE1C in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining UBE1C in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining UBE1C in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining UBE1C in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining UBE1C in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

  • Western blot analysis of UBE1C on different lysates with Rabbit anti-UBE1C antibody (<a href="/products/R1706-14" style="font-weight: bold;text-decoration: underline;">R1706-14</a>) at 1/1,000 dilution.<br /><br />Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate<br /><br />Exposure time: 3 minutes; ECL: K1802<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/R1706-14" style="font-weight: bold;text-decoration: underline;">R1706-14</a>, 1/,000 in 5% NFDM/TBST, 2 hour at room temperature<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 52 kDa<br />Observed band size: 52 kDa

    Western blot analysis of UBE1C on different lysates with Rabbit anti-UBE1C antibody (R1706-14) at 1/1,000 dilution.

    Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate

    Exposure time: 3 minutes; ECL: K1802

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: R1706-14, 1/,000 in 5% NFDM/TBST, 2 hour at room temperature
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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