Myosin Light Chain 2 Rabbit Polyclonal Antibody
Catalog# R1512-19
Myosin Light Chain 2 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Myosin Light Chain 2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human Myosin Light Chain 2 aa 21-70 / 166.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
Predicted band size: 19 kDa
Positive Control
A431 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse heart tissue lysate, Mouse skeletal tissue lysate, Rat heart tissue lysate, A431, C2C12, C6, mouse heart tissue, rat heart tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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IF-Cell
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1:1,000
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IHC-P
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1:50-1:200
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FC
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1:1,000
Target
Function
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC-2) also known as the regulatory light chain of myosin (RLC) is a protein that in humans is encoded by the MYL2 gene. This cardiac ventricular RLC isoform is distinct from that expressed in skeletal muscle (MYLPF), smooth muscle (MYL12B) and cardiac atrial muscle (MYL7). Ventricular myosin light chain-2 (MLC-2v) refers to the ventricular cardiac muscle form of myosin light chain 2 (Myl2). MLC-2v is a 19-KDa protein composed of 166 amino acids, that belongs to the EF-hand Ca2+ binding superfamily. MLC-2v interacts with the neck/tail region of the muscle thick filament protein myosin to regulate myosin motility and function.
Background References
1. Luo XL et al. Myosin light chain 2 marks differentiating ventricular cardiomyocytes derived from human embryonic stem cells. Pflugers Arch. 2021 Jul
2. Tsuji-Tamura K et al. Pharmacological control of angiogenesis by regulating phosphorylation of myosin light chain 2. Cell Signal. 2024 Aug
Post-translational Modification
N-terminus is methylated by METTL11A/NTM1.; Phosphorylated by MYLK3 and MYLK2; promotes cardiac muscle contraction and function (By similarity). Dephosphorylated by PPP1CB complexed to PPP1R12B (By similarity). The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation); regulates cardiac torsion and workload distribution (By similarity).
Subcellular Location
Cytoplasm.
Synonyms
Cardiac myosin light chain-2 antibody
Cardiac ventricular myosin light chain 2 antibody
CMH10 antibody
MLC 2v antibody
MLC-2 antibody
MLC-2v antibody
MLC2 antibody
MLRV_HUMAN antibody
MYL 2 antibody
MYL2 antibody
ExpandImages
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Western blot analysis of Myosin Light Chain 2 on different lysates with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/2,000 dilution.
Lane 1: A431 cell lysate (15 µg/Lane)
Lane 2: C2C12 cell lysate (15 µg/Lane)
Lane 3: C6 cell lysate (15 µg/Lane)
Lane 4: Mouse heart tissue lysate (20 µg/Lane)
Lane 5: Mouse skeletal tissue lysate (20 µg/Lane)
Lane 6: Rat heart tissue lysate (20 µg/Lane)
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 2 minutes 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-19) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A431 cells labeling Myosin Light Chain 2 with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of C2C12 cells labeling Myosin Light Chain 2 with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1.000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of C6 cells labeling Myosin Light Chain 2 with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of A431 cells labeling Myosin Light Chain 2.
Cells were fixed and permeabilized. Then stained with the primary antibody (R1512-19, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Myosin Light Chain 2 Recombinant Rabbit Monoclonal Antibody [SN67-09]
Application: WB,IHC-P,IP
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
Products with the same target and pathway
Myosin Light Chain 2 Recombinant Rabbit Monoclonal Antibody [SN67-09] - BSA and Azide free
Application: WB,IHC-P,IP
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated