MSH2 Rabbit Polyclonal Antibody
Catalog# R1510-32
MSH2 Rabbit Polyclonal Antibody
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IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MSH2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human MSH2 aa 21-70 / 934.
Species Reactivity
Human (Predicted: Mouse, Rat)
Predicted species support after-sales service
Validated Applications
IF-Cell, FC
Target Molecular Weight
Predicted band size: 105 kDa
Positive Control
Hela.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IF-Cell
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1:50-1:100
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FC
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1:50-1:100
Target
Function
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
Background References
1. Clinical significance of MLH1/MSH2 for stage II/III sporadic colorectal cancer. Wang SM. et. al. World J Gastrointest Oncol. 2019 Nov
2. A Whole-genome CRISPR Screen Identifies a Role of MSH2 in Cisplatin-mediated Cell Death in Muscle-invasive Bladder Cancer. Goodspeed A. et. al. Eur Urol. 2019 Feb
Sequence Similarity
Belongs to the DNA mismatch repair MutS family.
Tissue Specificity
Ubiquitously expressed.
Post-translational Modification
Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
Subcellular Location
Nucleus, Chromosome.
Synonyms
BAT26 antibody
COCA 1 antibody
COCA1 antibody
DNA mismatch repair protein Msh2 antibody
FCC 1 antibody
FCC1 antibody
hMSH2 antibody
HNPCC 1 antibody
HNPCC antibody
HNPCC1 antibody
ExpandImages
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ICC staining of MSH2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1510-32, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Flow cytometric analysis of MSH2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (R1510-32, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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