Skip to content

MSH2 Rabbit Polyclonal Antibody

Usd: 315

Specification

Bulk Order Quote | Customization Request

Catalog# R1510-32

MSH2 Rabbit Polyclonal Antibody

Application

  • IF-Cell

  • FC

Reactivity

  • Human

Predicted reactivity

  • Mouse

  • Rat

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

MSH2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Human MSH2 aa 21-70 / 934.

Species Reactivity

Human (Predicted: Mouse, Rat)

Predicted species support after-sales service

Validated Applications

IF-Cell, FC

Molecular Weight

Predicted band size: 105 kDa

Positive Control

Hela.

Conjugation

unconjugated

RRID

AB_3073340

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • IF-Cell

  • 1:50-1:100

  • FC

  • 1:50-1:100

Target

Function

This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.

Background References

1. Clinical significance of MLH1/MSH2 for stage II/III sporadic colorectal cancer. Wang SM. et. al. World J Gastrointest Oncol. 2019 Nov

2. A Whole-genome CRISPR Screen Identifies a Role of MSH2 in Cisplatin-mediated Cell Death in Muscle-invasive Bladder Cancer. Goodspeed A. et. al. Eur Urol. 2019 Feb

Sequence Similarity

Belongs to the DNA mismatch repair MutS family.

Tissue Specificity

Ubiquitously expressed.

Post-translational Modification

Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.

Subcellular Location

Nucleus, Chromosome.

UNIPROT

SwissProt: P43246 Human

SwissProt: P43247 Mouse

SwissProt: P54275 Rat

Synonyms

BAT26 antibody

COCA 1 antibody

COCA1 antibody

DNA mismatch repair protein Msh2 antibody

FCC 1 antibody

FCC1 antibody

hMSH2 antibody

HNPCC 1 antibody

HNPCC antibody

HNPCC1 antibody

Expand

Images

  • ICC staining of MSH2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/R1510-32" style="font-weight: bold;text-decoration: underline;">R1510-32</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of MSH2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1510-32, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Flow cytometric analysis of MSH2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/R1510-32" style="font-weight: bold;text-decoration: underline;">R1510-32</a>, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

    Flow cytometric analysis of MSH2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (R1510-32, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"