Gamma tubulin Rabbit Polyclonal Antibody
Catalog# R1411-2
Gamma tubulin Rabbit Polyclonal Antibody
-
WB
-
IF-Cell
-
IHC-P
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
Gamma tubulin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within C-terminal human Gamma tubulin.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 51 kDa
Positive Control
Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human heart tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1: 1,000
-
IF-Cell
-
1:50-1:100
-
IHC-P
-
1:50-1:100
Target
Function
This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7.
Background References
1. Chumová J. et. al. Microtubular and Nuclear Functions of gamma-Tubulin: Are They LINCed? Cells. 2019 Mar
2. Tovey CA. et. al. Microtubule nucleation by gamma-tubulin complexes and beyond. Essays Biochem. 2018 Dec
Sequence Similarity
Belongs to the tubulin family.
Post-translational Modification
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Subcellular Location
Cytoplasm, Cytoskeleton, Microtubule.
Synonyms
Gamma 1 tubulin antibody
Gamma 2 tubulin antibody
Gamma Tubulin 1 antibody
Gamma Tubulin 2 antibody
Gamma tubulin complex component 1 antibody
Gamma-2-tubulin antibody
GCP 1 antibody
GCP-1 antibody
GCP1 antibody
MGC131994 antibody
ExpandImages
-
Western blot analysis of Gamma tubulin on different lysates with Rabbit anti-Gamma tubulin antibody (R1411-2) at 1/1,000 dilution.
Lane 1: A431 (Human epidermal cancer cells) cell lysate
Lane 2: C2C12(Mouse myoblast) cell lysate
Lane 3: PC-12(Rat adrenal medulla pheochromocytoma cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 20s seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: R1411-2, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 51 kDa
Observed band size: 51 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
gamma Tubulin Recombinant Rabbit Monoclonal Antibody [JF0972]
Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC
Reactivity: Human,Mouse,Rat,Green monkey,Chicken,Hamster,Cow,Dog,Fish,Xenopus tropicalis
Conjugate: unconjugated
