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GFP Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1312-2

GFP Rabbit Polyclonal Antibody

Application

  • WB

  • ELISA

  • IP

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

GFP Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant full length protein of Aequorea victoria GFP.

Species Reactivity

Species independent

Validated Applications

WB, ELISA, IP

Molecular Weight

Predicted band size: 26 kDa

Positive Control

GFP protein

Conjugation

unconjugated

RRID

AB_3073265

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:10,000

  • ELISA

  • 1:10,000

  • IP

  • 2-5 µg/ml.

Target

Function

Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.

Background References

1. "Primary structure of the Aequorea victoria green-fluorescent protein."Prasher D.C., Eckenrode V.K., Ward W.W., Prendergast F.G., Cormier M.J.Gene 111:229-233(1992)

2. "A molecular thermometer based on fluorescent protein blinking."Wong F.H., Banks D.S., Abu-Arish A., Fradin C.J. Am. Chem. Soc. 129:10302-10303(2007)

Sequence Similarity

Belongs to the GFP family.

Tissue Specificity

Photocytes.

Post-translational Modification

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

UNIPROT

SwissProt: P42212 Aequorea victoria

Synonyms

GFP antibody

Green fluorescent protein antibody

yfp antibody

Images

  • Western blot analysis on GFP protein using rabbit polyclonal antibody.

    Western blot analysis on GFP protein using rabbit polyclonal antibody.

  • GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with <a href="/products/R1312-2" style="font-weight: bold;text-decoration: underline;">R1312-2</a> at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/M1004-8" style="font-weight: bold;text-decoration: underline;">M1004-8</a> at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:20,000 dilution was used for 60 mins at room temperature.<br /><br />Lane 1: GFP Tag fusion protein lysate (input).<br />Lane 2: Rabbit IgG instead of <a href="/products/R1312-2" style="font-weight: bold;text-decoration: underline;">R1312-2</a> in GFP Tag fusion protein lysate.<br />Lane 3: <a href="/products/ET1602-7" style="font-weight: bold;text-decoration: underline;">ET1602-7</a> IP in GFP Tag fusion protein lysate.<br />Lane 4: <a href="/products/ET1604-25" style="font-weight: bold;text-decoration: underline;">ET1604-25</a> IP in GFP Tag fusion protein lysate.<br />Lane 5: <a href="/products/ET1604-26" style="font-weight: bold;text-decoration: underline;">ET1604-26</a> IP in GFP Tag fusion protein lysate.<br />Lane 6: <a href="/products/ET1607-31" style="font-weight: bold;text-decoration: underline;">ET1607-31</a> IP in GFP Tag fusion protein lysate.<br />Lane 7: <a href="/products/R1312-2" style="font-weight: bold;text-decoration: underline;">R1312-2</a> IP in GFP Tag fusion protein lysate.<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST

    GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with R1312-2 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1004-8 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.

    Lane 1: GFP Tag fusion protein lysate (input).
    Lane 2: Rabbit IgG instead of R1312-2 in GFP Tag fusion protein lysate.
    Lane 3: ET1602-7 IP in GFP Tag fusion protein lysate.
    Lane 4: ET1604-25 IP in GFP Tag fusion protein lysate.
    Lane 5: ET1604-26 IP in GFP Tag fusion protein lysate.
    Lane 6: ET1607-31 IP in GFP Tag fusion protein lysate.
    Lane 7: R1312-2 IP in GFP Tag fusion protein lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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GFP Recombinant Rabbit Monoclonal Antibody [SY0243]

Application: WB,IF-Cell,IF-Tissue,IP,IHC-P,VIVIT

Reactivity: Species independent

Conjugate: unconjugated