Geminin Rabbit Polyclonal Antibody
Catalog# R1311-8
Geminin Rabbit Polyclonal Antibody
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IF-Cell
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IHC-P
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Mouse
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unconjugated
Overview
Product Name
Geminin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein with Mouse Geminin aa 70-206 / 206.
Species Reactivity
Mouse
Validated Applications
IF-Cell, IHC-P
Molecular Weight
Predicted band size: 23 kDa
Positive Control
F9, D3, human testis tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:100-1:200
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IF-Cell
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1:200
Target
Function
Geminin is a nuclear protein that is present in most eukaryotics and highly conserved across species. Numerous functions have been elucidated for Geminin including roles in metazoan cell cycle, cellular proliferation, cell lineage commitment, and neural differentiation. Geminin is absent during G1 phase and accumulates through S, G2 phase and M phases of the cell cycle. Geminin levels drop at the metaphase / anaphase transition of mitosis when it is degraded by the Anaphase Promoting Complex (APC/C). During S phase, geminin is a positive regulator of DNA replication. In many cancer cell lines, inhibition of geminin by RNAi results in re-replication of portions of the genome, which leads to aneuploidy. In these cell lines, geminin knockdown leads to markedly slowed growth and apoptosis within several days. Recently, geminin has been found to be overexpressed in several malignancies and cancer cell lines.
Background References
1. "Mouse geminin inhibits not only Cdt1-MCM6 interactions but also a novel intrinsic Cdt1 DNA binding activity." Yanagi K., Mizuno T., You Z., Hanaoka F. J. Biol. Chem. 277:40871-40880(2002)
2. "Structural basis for inhibition of the replication licensing factor Cdt1 by geminin." Lee C., Hong B., Choi J.M., Kim Y., Watanabe S., Ishimi Y., Enomoto T., Tada S., Kim Y., Cho Y. Nature 430:913-917(2004)
Sequence Similarity
Belongs to the geminin family.
Post-translational Modification
Phosphorylated during mitosis. Phosphorylation at Ser-181 by CK2 results in enhanced binding to Hox proteins and more potent inhibitory effect on Hox transcriptional activity.
Subcellular Location
Cytoplasm, nucleus
UNIPROT
Synonyms
DNA replication inhibitor antibody
Gem antibody
GEMI_HUMAN antibody
Geminin antibody
Geminin DNA replication inhibitor antibody
GMNN antibody
OTTHUMP00000039393 antibody
RP3 369A17.3 antibody
Images
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ICC staining Geminin in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
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ICC staining Geminin in D3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
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Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Geminin antibody (R1311-8) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1311-8) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"