Active+Pro Caspase-9 Rabbit Polyclonal Antibody (R1308-12)
Catalog# R1308-12
Rabbit Polyclonal to Active+Pro Caspase-9
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WB
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IHC-P
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Human
Western blot analysis of Active+Pro Caspase-9 on different lysates with Rabbit anti-Active+Pro Caspase-9 antibody (R1308-12) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HeLa treated with 1μM staurosporine for 4 hours cell lysate (20 µg/Lane)
Lane 3: HeLa treated with 3μM staurosporine for 4 hours cell lysate (20 µg/Lane)
Predicted band size: 46 kDa
Observed band size: 46/37/35 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-12) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Active+Pro Caspase-9 Rabbit Polyclonal Antibody (R1308-12)
Immunogen
Synthetic peptide within human caspase-9 aa 299-313/416.
Host
Rabbit
Positive Control
HeLa cell lysate, HeLa treated with 1μM staurosporine for 4 hours cell lysate, HeLa treated with 3μM staurosporine for 4 hours cell lysate, human lung carcinoma tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000- 1:2,000
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IHC-P
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 46 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Active+Pro Caspase-9
SYNONYMS
Caspase9;APAF-3 antibody;APAF3 antibody;Apoptosis related cysteine peptidase antibody;Apoptotic protease Mch-6 antibody;Apoptotic protease-activating factor 3 antibody;CASP-9 antibody;CASP9 antibody;CASP9_HUMAN antibody;Caspase 9 apoptosis related cysteine peptidase antibody;Caspase 9 Dominant Negative antibody;Caspase 9c antibody;Caspase-9 antibody;Caspase-9 subunit p10 antibody;ICE LAP6 antibody;ICE like apoptotic protease 6 antibody;ICE-LAP6 antibody;ICE-like apoptotic protease 6 antibody;MCH6 antibody;PPP1R56 antibody;protein phosphatase 1, regulatory subunit 56 antibody;RNCASP9 antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase C14A family.
TISSUE SPECIFICITY
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
DEVELOPMENTAL STAGE
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
POST-TRANSLATIONAL MODIFICATION
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.; Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. It is known to be involved in the activation cascade of caspases responsible for apoptosis execution. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response. Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis.
CITATIONS
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Benzo [b] fluoranthene induced oxidative stress and apoptosis in human airway epithelial cells via mitochondrial disruption
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WB
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Human
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Citation
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Fan Chengcheng,et al
Natural Product-Inspired Discovery of Naphthoquinone-Furo-Piperidine Derivatives as Novel STAT3 Inhibitors for the Treatment of Triple-Negative Breast Cancer
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WB
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Human
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Citation
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Liu, X., Cui, S., Dan, C., Li, W., Xie, H., Li, C., & Shi, L.
Phellinus baumii Polyphenol: A Potential Therapeutic Candidate against Lung Cancer Cells
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WB
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Human
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Citation
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