VMAT1 Rabbit Polyclonal Antibody
Catalog# R1307-7
VMAT1 Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
VMAT1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human VMAT1 aa 91-140 / 525.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 56 kDa
Positive Control
SH-SY5Y cell lysate, PC-12 cell lysate, Neuro-2a cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:2,000
Target
Function
Vesicular monoamine transporter 1 (VMAT1) also known as chromaffin granule amine transporter (CGAT) or solute carrier family 18 member 1 (SLC18A1) is a protein that in humans is encoded by the SLC18A1 gene. VMAT1 is an integral membrane protein, which is embedded in synaptic vesicles and serves to transfer monoamines, such as norepinephrine, epinephrine, dopamine, and serotonin, between the cytosol and synaptic vesicles. SLC18A1 is an isoform of the vesicular monoamine transporter.
Background References
1. Anlauf M, Eissele R, Sch fer MK, Eiden LE, Arnold R, Pauser U, Kl ppel G, Weihe E (August 2003). "Expression of the two isoforms of the vesicular monoamine transporter (VMAT1 and VMAT2) in the endocrine pancreas and pancreatic endocrine tumors". J. Histochem. Cytochem. 51 (8): 1027–40.
2. Lohoff F, Dahl J, Ferraro T, Arnold S, Gallinat J, Sander T, Berrettini W (December 2006). "Variations in the vesicular monoamine transporter type 1 gene (VMAT1/SLC18A1) are associated with bipolar I disorder". Neuropsychopharmacology 31 (12): 2739–2747.
Sequence Similarity
Belongs to the major facilitator superfamily. Vesicular transporter family.
Tissue Specificity
Highly expressed in chromaffin cells of the adrenal medulla (at protein level). Detected in peripheral sympathetic ganglia (at protein level). Found in some paracrine cells in stomach and duodenum (at protein level).
Subcellular Location
Cytoplasmic vesicle membrane; Endoplasmic reticulum membrane
Synonyms
4832416I10Rik antibody
Adrenal chromaffin granule amine transporter
CGAT antibody
CGAT antibody
Chromaffin granule amine transporter antibody
MGC28683 antibody
MGC37299 antibody
SLC18A1 antibody
Solute carrier family 18 (vesicular monoamine), member 1 antibody
Solute carrier family 18 member 1 antibody
ExpandImages
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Western blot analysis of VMAT1 on different lysates with Rabbit anti-VMAT1 antibody (R1307-7) at 1/2,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Neuro-2a cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 51 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-7) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"