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6X His tag Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1207-2

6X His tag Rabbit Polyclonal Antibody

Application

  • WB

  • IP

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

6X His tag Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide immune sequence is HHHHHHHHC.

Species Reactivity

Species independent

Validated Applications

WB, IP

Positive Control

C-terminal His-tagged recombinant protein lysates.

Conjugation

unconjugated

RRID

AB_3073202

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IP

  • 2-5 µg/ml.

Target

Function

His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Background References

暂无

Synonyms

6 His epitope tag antibody

Hexa His tag antibody

HHHHHH epitope tag antibody

HHHHHH tag antibody

His tag antibody

Polyhistidine Tag antibody

Images

  • Western blot analysis of 6X His tag on C-terminal His-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/R1207-2" style="font-weight: bold;text-decoration: underline;">R1207-2</a>, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of 6X His tag on C-terminal His-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1207-2, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • His tag was immunoprecipitated in 2µg C terminal His Tag fusion protein lysate with <a href="/products/R1207-2" style="font-weight: bold;text-decoration: underline;">R1207-2</a> at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/M0812-3" style="font-weight: bold;text-decoration: underline;">M0812-3</a> at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:20,000 dilution was used for 60 mins at room temperature.<br /><br />Lane 1: His Tag fusion protein lysate (input).<br />Lane 2: 0812-1 IP in His Tag fusion protein lysate.<br />Lane 3: <a href="/products/R1207-2" style="font-weight: bold;text-decoration: underline;">R1207-2</a> IP in His Tag fusion protein lysate.<br />Lane 4: Rabbit IgG instead of <a href="/products/R1207-2" style="font-weight: bold;text-decoration: underline;">R1207-2</a> in His Tag fusion protein lysate.<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST

    His tag was immunoprecipitated in 2µg C terminal His Tag fusion protein lysate with R1207-2 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M0812-3 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.

    Lane 1: His Tag fusion protein lysate (input).
    Lane 2: 0812-1 IP in His Tag fusion protein lysate.
    Lane 3: R1207-2 IP in His Tag fusion protein lysate.
    Lane 4: Rabbit IgG instead of R1207-2 in His Tag fusion protein lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation