Geminin Rabbit Polyclonal Antibody
Catalog# R1204-2
Geminin Rabbit Polyclonal Antibody
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Geminin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human Geminin aa 1-50 / 209.
Species Reactivity
Human, Mouse (Predicted: Rat)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
24 kDa
Positive Control
Hela cell lysates, NCCIT, F9, HepG2.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:50
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FC
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1:50-1:100
Target
Function
Geminin is a nuclear protein that is present in most eukaryotics and highly conserved across species. Numerous functions have been elucidated for Geminin including roles in metazoan cell cycle, cellular proliferation, cell lineage commitment, and neural differentiation. During S phase, geminin is a negative regulator of DNA replication. In many cancer cell lines, inhibition of geminin by RNAi results in re-replication of portions of the genome, which leads to aneuploidy.
Background References
1. Pitulscu M,Kessel M,Luo L.The regulation of embryonic patterning and DNA replication by geminin. Cell Mol Life Sci,2005,62 (13):1425～1433.
2. Achibana K E,Gonzalez M A,Guarguaglini G,et al.Depletion of licensing inhibitor geminin causes centrosome overduplication and mitotic defects. EMBO Rep,2005,6 (11):1052～1057.
3. Kulartz M,Knippers R.The replicative regulator protein geminin on chromatin in the HeLa cell cycle.J Biol Chem,2004,279 (40):41686～41694.
Sequence Similarity
Belongs to the geminin family.
Post-translational Modification
Phosphorylated during mitosis. Phosphorylation at Ser-184 by CK2 results in enhanced binding to Hox proteins and more potent inhibitory effect on Hox transcriptional activity.
Subcellular Location
Nucleus, cytoplasm
Synonyms
DNA replication inhibitor antibody
Gem antibody
GEMI_HUMAN antibody
Geminin antibody
Geminin DNA replication inhibitor antibody
GMNN antibody
OTTHUMP00000039393 antibody
RP3 369A17.3 antibody
Images
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Western blot analysis of Geminin on different lysates with Rabbit anti-Geminin antibody (R1204-2) at 1/1,000 dilution.
Lane 1: NCCIT
Lane 2: HeLa
Lane 2: Hep G2
Lysates/proteins at 20 µg/Lane.
Exposure time: 6 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: R1204-2, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 24 kDa
Observed band size: 30 kDa -
ICC staining of Geminin in NCCIT cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1204-2, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of Geminin in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1204-2, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Flow cytometric analysis of Geminin was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (R1204-2, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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