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NOX2 / gp91phox Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1511-34

NOX2 / gp91phox Rabbit Polyclonal Antibody

Application

  • IF-Cell

  • IHC-P

  • FC

  • WB

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

NOX2 / gp91phox Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide corresponding to C-terminal human NOX2.

Species Reactivity

Human, Mouse

Validated Applications

IF-Cell, IHC-P, FC, WB

Molecular Weight

Predicted band size: 65 kDa

Positive Control

RAW264.7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, RAW264.7, human liver tissue, human spleen tissue, human tonsil tissue, mouse spleen tissue.

Conjugation

unconjugated

RRID

AB_3069031

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

  • WB

  • 1:2,000

Target

Function

Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet they have distinct expression patterns. gp91-phox is expressed in eosinophils, neutro-phils, monocytes and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, and low expression is also detected in the uterus and prostate. Mox1 is also upregulated in vascular smooth-muscle cells in response to PDGF stimulation, which collectively indicates that Mox1 may function analogously to gp91-phox, yet regulate the NADPH superoxide production in non-phagocytic cells.

Background References

暂无

Tissue Specificity

Detected in neutrophils (at protein level).

Post-translational Modification

Glycosylated.; Phosphorylated on Ser and Thr residues.; Undergoes 'Lys-48'-linked polyubiquitination, likely by RNF145, triggering endoplasmic reticulum-associated degradation.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P04839 Human

SwissProt: Q61093 Mouse

Synonyms

AMCBX2 antibody

CGD antibody

CGD91-phox antibody

CY24B_HUMAN antibody

CYBB antibody

Cytochrome b 245, beta polypeptide antibody

Cytochrome b(558) beta chain antibody

Cytochrome b(558) subunit beta antibody

Cytochrome b-245 heavy chain antibody

Cytochrome b558 subunit beta antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of NOX2 / gp91phox on different lysates with Rabbit anti-NOX2 / gp91phox antibody (<a href="/products/ER1511-34" style="font-weight: bold;text-decoration: underline;">ER1511-34</a>) at 1/2,000 dilution.<br /><br />Lane 1: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 2: Mouse spleen tissue lysate (40 µg/Lane)<br />Lane 3: Mouse skeletal muscle tissue lysate (negative) (40 µg/Lane)<br />Lane 4: Rat spleen tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 65 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1511-34" style="font-weight: bold;text-decoration: underline;">ER1511-34</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of NOX2 / gp91phox on different lysates with Rabbit anti-NOX2 / gp91phox antibody (ER1511-34) at 1/2,000 dilution.

    Lane 1: RAW264.7 cell lysate (20 µg/Lane)
    Lane 2: Mouse spleen tissue lysate (40 µg/Lane)
    Lane 3: Mouse skeletal muscle tissue lysate (negative) (40 µg/Lane)
    Lane 4: Rat spleen tissue lysate (40 µg/Lane)

    Predicted band size: 65 kDa
    Observed band size: 55 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1511-34) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of RAW264.7 cells labeling NOX2 / gp91phox with Rabbit anti-NOX2 / gp91phox antibody (<a href="/products/ER1511-34" style="font-weight: bold;text-decoration: underline;">ER1511-34</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NOX2 / gp91phox antibody (<a href="/products/ER1511-34" style="font-weight: bold;text-decoration: underline;">ER1511-34</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of RAW264.7 cells labeling NOX2 / gp91phox with Rabbit anti-NOX2 / gp91phox antibody (ER1511-34) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NOX2 / gp91phox antibody (ER1511-34) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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