NOX2 / gp91phox Rabbit Polyclonal Antibody (ER1511-34)
Catalog# ER1511-34
Rabbit Polyclonal to NOX2/gp91phox
Tested for
-
IF-Cell
-
IHC-P
-
FC
-
WB
Reacts with
-
Human
-
Mouse
☑ Relative expression (RE)
Western blot analysis of NOX2 / gp91phox on different lysates with Rabbit anti-NOX2 / gp91phox antibody (ER1511-34) at 1/2,000 dilution.
Lane 1: RAW264.7 cell lysate (20 µg/Lane)
Lane 2: Mouse spleen tissue lysate (40 µg/Lane)
Lane 3: Mouse skeletal muscle tissue lysate (negative) (40 µg/Lane)
Lane 4: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 65 kDa
Observed band size: 55 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1511-34) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NOX2 / gp91phox on different lysates with Rabbit anti-NOX2 / gp91phox antibody (ER1511-34) at 1/2,000 dilution.
Lane 1: RAW264.7 cell lysate (20 µg/Lane)
Lane 2: Mouse spleen tissue lysate (40 µg/Lane)
Lane 3: Mouse skeletal muscle tissue lysate (negative) (40 µg/Lane)
Lane 4: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 65 kDa
Observed band size: 55 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1511-34) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.