MAL Mouse Monoclonal Antibody [B5-G3]
Catalog# M1701-6
MAL Mouse Monoclonal Antibody [B5-G3]
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MAL Mouse Monoclonal Antibody [B5-G3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within mouse MAL aa 97-146 / 153.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 17 kDa
Positive Control
Mouse kidney tissue, mouse lymphatic vessels tissue lysate, rat brain tissue, rat kidney tissue, mouse brain tissue, Jurkat.
Conjugation
unconjugated
Clone Number
B5-G3
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:600
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FC
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1:50-1:200
Target
Function
MAL (myelin and lymphocyte protein), also known as T lymphocyte maturation-associated protein, is a nonglycosylated hydrophobic integral membrane protein belonging to the MAL family of proteolipids. MAL is highly enriched in nervous system myelin and in rafts and apical membranes of epithelial cells. It is involved in forming, stabilizing and maintaining glycosphingolipid-enriched membrane microdomains. MAL maintains the myelin sheath and, by controlling the sorting and trafficking of oligodendrocytes, it is involved in central nervous system paranode maintenance. MAL is a component of lipid rafts in myelinating cells. Association with glycosphingolipids may result in protein-lipid microdomain formation in myelin. MAL has been localized to the endoplasmic reticulum of T cells and in compact myelin of cells in the nervous system. MAL is primarily expressed by oligodendrocytes and Schwann cells in the intermediate and late stages of T cell differentiation.
Background References
1. Carmosino M et al. MAL/VIP17, a new player in the regulation of NKCC2 in the kidney. Mol Biol Cell 21:3985-97 (2010).
2. Kamsteeg EJ et al. MAL decreases the internalization of the aquaporin-2 water channel. Proc Natl Acad Sci U S A 104:16696-701 (2007).
Sequence Similarity
Belongs to the MAL family.
Post-translational Modification
Lipoprotein.
Subcellular Location
Membrane.
Synonyms
mal antibody
MAL protein gene antibody
Mal T-cell differentiation protein antibody
MAL_HUMAN antibody
MALGENE antibody
MPV17 antibody
Myelin and lymphocyte protein antibody
T-cell differentiation protein MAL antibody
T-lymphocyte maturation-associated protein antibody
VIP17 antibody
Images
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Western blot analysis of MAL on Mouse kidney tissue lysate with Mouse anti-MAL antibody (M1701-6) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 100 seconds; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: M1701-6, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/5,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 17 kDa
Observed band size: 25 kDa -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-MAL antibody (M1701-6) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-6) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-MAL antibody (M1701-6) at 1/1000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-6) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-MAL antibody (M1701-6) at 1/1000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-6) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"