gamma tubulin Mouse Monoclonal Antibody [10-A4]
Catalog# M1701-13
gamma tubulin Mouse Monoclonal Antibody [10-A4]
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
gamma tubulin Mouse Monoclonal Antibody [10-A4]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human gamma tubulin aa 402-451 / 451.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell
Target Molecular Weight
Predicted band size: 51 kDa
Positive Control
Rat epididymis tissue, human tonsil tissue, human colon cancer tissue, mouse testis tissue.
Conjugation
unconjugated
Clone Number
10-A4
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IF-Cell
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1:100-1:500
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IHC-P
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1:50-1:200
Target
Function
Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.
Background References
1. Morris EJ et al. Stat3 regulates centrosome clustering in cancer cells via Stathmin/PLK1. Nat Commun 8:15289 (2017).
2. Goldspink DA et al. Ninein is essential for apico-basal microtubule formation and CLIP-170 facilitates its
Sequence Similarity
Belongs to the tubulin family.
Post-translational Modification
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Subcellular Location
Cytoplasm, cytoskeleton, centrosome.
Synonyms
Gamma 1 tubulin antibody
Gamma 2 tubulin antibody
Gamma Tubulin 1 antibody
Gamma Tubulin 2 antibody
Gamma tubulin complex component 1 antibody
Gamma-2-tubulin antibody
GCP 1 antibody
GCP1 antibody
MGC131994 antibody
TBG2_HUMAN antibody
ExpandImages
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Western blot analysis of gamma tubulin on different lysates with Rabbit anti-gamma tubulin antibody (M1701-13) at 1/2,000 dilution.
Lane 1: Jurkat
Lane 2: U-2 OS
Lane 3: NIH/3T3
Lane 4: C2C12
Lane 5: C6
Lane 6: PC-12
Lane 7: COS-7
Lysates/proteins at 20 µg/Lane.
Exposure time: 15 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: M1701-13, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 51.2kDa
Observed band size: 50 kDa -
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.
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Immunocytochemistry analysis of Hela cells labeling gamma tubulin with Mouse anti-gamma tubulin antibody (M1701-13) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at RT, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-gamma tubulin antibody (M1701-13) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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