Lamin B2 Mouse Monoclonal Antibody [D5-C2]
Catalog# M1505-11
Lamin B2 Mouse Monoclonal Antibody [D5-C2]
-
WB
-
IF-Cell
-
IHC-P
-
Human
-
Mouse
-
Rat
-
Zebrafish
-
unconjugated
Overview
Product Name
Lamin B2 Mouse Monoclonal Antibody [D5-C2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human Lamin B2 aa 571-620 / 620.
Species Reactivity
Human, Mouse, Rat, Zebrafish
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 70 kDa
Positive Control
Hela cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, hybrid fish (crucian-carp) brain tissue lysates, human uterine tissue.
Conjugation
unconjugated
Clone Number
D5-C2
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2b
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000-1:5,000
-
IF-Cell
-
1:200-1:500
-
IHC-P
-
1:500-1:1000
Target
Function
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Background References
1. "Sequencing of the reannotated LMNB2 gene reveals novel mutations in patients with acquired partial lipodystrophy." Hegele R.A., Cao H., Liu D.M., Costain G.A., Charlton-Menys V., Rodger N.W., Durrington P.N. Am. J. Hum. Genet. 79:383-389(2006)
2. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011)
Sequence Similarity
Belongs to the intermediate filament family.
Post-translational Modification
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Subcellular Location
Nucleus inner membrane
UNIPROT
Synonyms
Lamin-B2 antibody
Lamin B2 antibody
LMN 2 antibody
LMN B2 antibody
LMN2 antibody
LMNB 2 antibody
LMNB2 antibody
LMNB2_HUMAN antibody
MGC2721 antibody
RGD1563803 antibody
Images
-
Western blot analysis of Lamin B2 on different lysates with Mouse anti-Lamin B2 antibody (M1505-11) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 3: C6 (Rat glioma cell) cell lysate
Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 12 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: M1505-11, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 70 kDa
Observed band size: 70 kDa -
Western blot analysis of Lamin B2 on zebrafish tissue lysate using anti-Lamin B2 antibody at 1/500 dilution.
-
Immunohistochemical analysis of paraffin-embedded human uterine tissue with Mouse anti-Lamin B2 antibody (M1505-11) at 1/1000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-11) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Lamin B2 Recombinant Mouse Monoclonal Antibody [J9-C4-R]
Application: WB,IF-Cell,IHC-P,IF-Tissue
Reactivity: Human,Green monkey
Conjugate: unconjugated
Products with the same target and pathway
Lamin B2 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Lamin B2 Mouse Monoclonal Antibody [J9-C4]
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
