MASP2 Mouse Monoclonal Antibody [F3-4]
Catalog# M1501-11
MASP2 Mouse Monoclonal Antibody [F3-4]
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WB
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Human
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unconjugated
Overview
Product Name
MASP2 Mouse Monoclonal Antibody [F3-4]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within human MASP2 aa 335-379/686.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 76 kDa
Positive Control
Human serum
Conjugation
unconjugated
Clone Number
F3-4
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2b
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
Target
Function
Mannan-binding lectin serine protease 2 also known as mannose-binding protein-associated serine protease 2 (MASP-2) is an enzyme that in humans is encoded by the MASP2 gene. The Ra-reactive factor (RARF) is a complement-dependent bactericidal factor that binds to the Ra and R2 polysaccharides expressed by certain enterobacteria. Alternate splicing of this gene results in two transcript variants encoding two RARF components that are involved in the mannan-binding lectin pathway of complement activation. The longer isoform is cleaved into two chains which form a heterodimer linked by a disulfide bond. The encoded proteins are members of the trypsin family of peptidases. MASP-2 is involved in the complement system. MASP-2 is very similar to the C1s molecule, of the classical complement pathway, and they are thought to have a common evolutionary ancestor. When the carbohydrate-recognising heads of MBL bind to specifically arranged mannose residues on the surface of a pathogen, MASP-2 is activated to cleave complement components C4 and C2 into C4a, C4b, C2a, and C2b.
Background References
1. Elhadad S et al. MASP2 inhibition by narsoplimab suppresses endotheliopathies characteristic of transplant-associated thrombotic microangiopathy: in vitro and ex vivo evidence. Clin Exp Immunol. 2023 Jul
2. Li Z et al. Impact of MASP2 gene polymorphism and gene-tea drinking interaction on susceptibility to tuberculosis. Sci Rep. 2021 Mar
Sequence Similarity
Belongs to the ku70 family.
Post-translational Modification
Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.; ADP-ribosylated by PARP3.
Subcellular Location
Secreted.
UNIPROT
Synonyms
Mannan binding lectin serine peptidase 2 antibody
Mannan binding lectin serine protease 2 antibody
Mannose binding protein associated serine protease 2 antibody
MAP 19 antibody
MAP19 antibody
MASP 2 antibody
MBL associated plasma protein of 19 kD antibody
MBL associated serine protease 2 antibody
Small MBL associated protein antibody
sMAP antibody
Images
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Western blot analysis on human serum lysates using anti-MASP2 mouse mAb.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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