DYKDDDDK Tag (FLAG) Mouse Monoclonal Antibody [A2-A4] (M1403-2)
Catalog# M1403-2
Mouse Monoclonal to DYKDDDDK Tag (FLAG)
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WB
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IP
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ELISA
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IF-Cell
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Species independent
Predicted band size: 35 kDa;
Observed band size: 35 kDa;
Exposure time: 60 seconds;
SDS-PAGE gel concentration:12%
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1403-2, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
DYKDDDDK Tag (FLAG) Mouse Monoclonal Antibody [A2-A4] (M1403-2)
Immunogen
Synthetic peptide immune sequence is n-dykddddk-c.
Host
Mouse
Positive Control
DYKDDDDK Tag recombinant protein, C-terminal FLAG-tagged recombinant protein, N-terminal FLAG-tagged recombinant protein.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A2-A4
RRID
APPLICATION DILUTION
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WB
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1:5,000-1:10,000
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IP
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2-5 µg/ml.
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Isotype
IgG1
TARGET
PROTEIN NAME
DYKDDDDK Tag (FLAG)
SYNONYMS
DDDDK epitope tag antibody;DDDK antibody;ddk antibody;DYKDDDDK antibody;DYKDDDDK epitope tag antibody;DYKDDDDK tag antibody;ECS epitope tag antibody;ECS tag antibody;Enterokinase Cleavage Site epitope tag antibody;Enterokinase Cleavage Site tag antibody;FLAG antibody;FLAG tag antibody
FUNCTION
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence, immunoprecipitation or detection by SDS PAGE protein electrophoresis and Western blotting. The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). Additionally, it may be used in tandem, commonly the 3xFLAG peptide: DYKDHD-G-DYKDHD-I-DYKDDDDK (with the final tag encoding an enterokinase cleavage site). It can be fused to the C-terminus or the N-terminus of a protein, or inserted within a protein. The tyrosine residue in the FLAG-tag can be sulfated, which can affect antibody recognition of the FLAG epitope. The FLAG-tag can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag.
CITATIONS
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Wang, J., Huang, Z., Huang, P., Wang, Q., Li, Y., Liu, X. H., Lin, F. C., & Lu, J.
The Plant Homeodomain Protein Clp1 Regulates Fungal Development, Virulence, and Autophagy Homeostasis in Magnaporthe oryzae
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WB
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Co-IP
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Magnaporthe oryzae
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Jiang, J., Li, Y., Sun, Z., Gong, L., Li, X., Shi, F., Yao, J., Meng, Y., Meng, X., Zhang, Q., Wang, Y., Su, X., & Diao, H.
LncNSPL facilitates influenza A viral immune escape by restricting TRIM25-mediated K63-linked RIG-I ubiquitination
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WB
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Yan, Y. Q., Zheng, R., Liu, Y., Ruan, Y., Lin, Z. H., Xue, N. J., Chen, Y., Zhang, B. R., & Pu, J. L.
Parkin regulates microglial NLRP3 and represses neurodegeneration in Parkinson's disease
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IF
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Mouse
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Huang, P., Li, Y., Wang, J., Wang, Q., Huang, Z., Liu, X., Lin, F., & Lu, J.
Casein Kinase 2 Mediates Degradation of Transcription Factor Pcf1 during Appressorium Formation in the Rice Blast Fungus
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Co-IP
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M.oryzae
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Zhou, D., Yan, H., Yang, S., Zhang, Y., Xu, X., Cen, X., Lei, K., & Xia, H.
SC75741, A Novel c-Abl Inhibitor, Promotes the Clearance of TDP25 Aggregates via ATG5-Dependent Autophagy Pathway
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WB
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Human
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Jiyong Zhou,Jinyan Gu
Identification and function analysis of canine stimulator of interferon gene (STING)
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WB
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dogs
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MDCK cells
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293T cells and A549 cells
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Jiemin Wong
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IF
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human
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The kinase CIPK11 functions as a positive regulator in cadmium stress response in Arabidopsis
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WB
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Wang, K., Huang, W., Chen, R., Lin, P., Zhang, T., Ni, Y. F., Li, H., Wu, J., Sun, X. X., Geng, J. J., Zhu, Y. M., Nan, G., Zhang, W., Chen, X., Zhu, P., Bian, H., & Chen, Z. N.
Di-methylation of CD147-K234 promotes the progression of NSCLC by enhancing lactate export
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IP
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Human
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Citation
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Yang, Y., Song, L., Huang, X., Feng, Y., Zhang, Y., Liu, Y., Li, S., Zhan, Z., Zheng, L., Feng, H., & Li, Y.
PRPS1-mediated purine biosynthesis is critical for pluripotent stem cell survival and stemness. Aging, 13(3), 4063–4078.
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WB
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Human
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Citation
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Wei, X., Zheng, Z., Feng, Z., Zheng, L., Tao, S., Zheng, B., Huang, B., Zhang, X., Liu, J., Chen, Y., Zong, W., Shan, Z., Fan, S., Chen, J., & Zhao, F.
Sigma-1 receptor attenuates osteoclastogenesis by promoting ER-associated degradation of SERCA2
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Co-IP
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Mouse
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Citation
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Zebrafish SETD3 mediated ubiquitination of phosphoprotein limits spring viremia of carp virus infection.
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Zebrafish
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Citation
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Chen, R., Huang, M., Yang, X., Chen, X. H., Shi, M. Y., Li, Z. F., Chen, Z. N., & Wang, K.
CALR-TLR4 Complex Inhibits Non-Small Cell Lung Cancer Progression by Regulating the Migration and Maturation of Dendritic Cells
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WB
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Mouse
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Citation
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He, Y., Wang, X., Lu, W., Zhang, D., Huang, L., Luo, Y., Xiong, L., Li, H., Zhang, P., Li, Q., & Liang, S.
PGK1 contributes to tumorigenesis and sorafenib resistance of renal clear cell carcinoma via activating CXCR4/ERK signaling pathway and accelerating glycolysis
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WB
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Human
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Citation
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Jin, X., Li, L., Peng, Q., Gan, C., Gao, L., He, S., Tan, S., Pu, W., Liu, Y., Gong, Y., Yao, Y., Wang, G., Liu, X., Gong, M., Lei, P., Zhang, H., Qi, S., Xu, H., Hu, H., Dong, B., … Dai, L.
Glycyrrhetinic acid restricts mitochondrial energy metabolism by targeting SHMT2
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WB
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Human
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Citation
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Li, X., Li, H., Jing, Q., Wang, M., Hu, T., Li, L., Zhang, Q., Liu, M., Fu, Y. V., Han, J., & Su, D.
Structural insights into multifunctionality of human FACT complex subunit hSSRP1
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IP
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Citation
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Li, J., Wang, K., Zhang, Y., Qi, T., Yuan, J., Zhang, L., Qiu, H., Wang, J., Yang, H. T., Dai, Y., Song, Y., & Chang, X.
Therapeutic Exon Skipping via a CRISPR-guided Cytidine Deaminase Rescues Dystrophic Cardiomyopathy In Vivo
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WB
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Mouse
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Citation
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Qin Liu
The Edwardsiella piscicida thioredoxin-like protein inhibits ASK1-MAPKs signaling cascades to promote pathogenesis during infection.
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WB
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HEK293T
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Citation
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Qu, Y., Wang, J., Huang, P., Liu, X., Lu, J., & Lin, F. C.
PoRal2 Is Involved in Appressorium Formation and Virulence via Pmk1 MAPK Pathways in the Rice Blast Fungus Pyricularia oryzae
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Co-IP
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Citation
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Gao, Y., Xiang, D., Li, W., Zheng, X., Wang, L., Li, Z., & Chen, T.
BRAFV600E Mutation-Responsive miRNA-222-3p Promotes Metastasis of Papillary Thyroid Cancer Cells via Snail-Induced EMT
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WB
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Citation
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Jie Wu ;Ayaz Najafov;Hongguang Xia
Pharmacological targeting of MCL-1 promotes mitophagy and improves disease pathologies in an Alzheimer’s disease mouse model
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WB
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COIP
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human
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Citation
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Kai Wang
Human tumor necrosis factor (TNF)-alpha-induced protein 8-like 2 suppresses hepatocellular carcinoma metastasis through inhibiting Rac1
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Co-IP
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human
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Citation
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Huang, W., Zhao, X., Ji, N., Guo, J., Feng, J., Chen, K., Wu, Y., Wang, J., Feng, H., & Zou, J.
IRF2 Cooperates with Phosphoprotein of Spring Viremia of Carp Virus to Suppress Antiviral Response in Zebrafish
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WB
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Zebrafish
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Citation
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Dahai Yang,Qin Liu
Novel T3SS effector EseK in Edwardsiella piscicida is chaperoned by EscH and EscS to express virulence
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WB
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HeLa cells
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Cai, Y. Y., Wang, J. Y., Wu, X. Y., Liang, S., Zhu, X. M., Li, L., Lu, J. P., Liu, X. H., & Lin, F. C.
MoOpy2 is essential for fungal development, pathogenicity, and autophagy in Magnaporthe oryzae
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Magnaporthe oryzae
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Citation
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Qian, H., Sun, L., Wu, M., Zhao, W., Liu, M., Liang, S., Zhu, X., Li, L., Su, Z., Lu, J., Lin, F., & Liu, X.
The COPII subunit MoSec24B is involved in development, pathogenicity and autophagy in the rice blast fungus
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WB
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M.oryzae
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Citation
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Cand2 Inhibits CRL-mediated Ubiquitination and Suppresses Autophagy to Facilitate Pathogenicity of Phytopathogenic Fungi
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WB
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Ustilaginoidea virens
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Di Wang
Bile Acids Control Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome
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WB
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human
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Citation
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Xueli Bai * Tingbo Liang
Primary tumor-derived exosomes facilitate metastasis by regulating adhesion of circulating tumor cells via SMAD3 in liver cancer
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IF
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Human
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Citation
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Wang, Q., Wang, J., Huang, P., Huang, Z., Li, Y., Liu, X., Lin, F., & Lu, J.
Nucleosome Assembly Protein 1, Nap1, Is Required for the Growth, Development, and Pathogenicity of Magnaporthe oryzae
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WB
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Citation
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Guoqiang Xu
A Mental Retardation-linked Nonsense Mutation in Cereblon Is Rescued by Proteasome Inhibition
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IF
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WB
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human
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Citation
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A VASt-domain protein regulates autophagy, membrane tension, and sterol homeostasis in rice blast fungus
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Co-IP
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Citation
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Guoqiang Xu
Quantitative proteomics and biochemical analyses reveal the role of endoplasmin in the regulation of the expression and secretion of A Disintegrin And Metalloproteinase 12
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WB
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HeLa cells
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Citation
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Haruhiko Koseki& Jiemin Wong
UHRF1 targets DNMT1 for DNA methylation through cooperative binding of hemi-methylated DNA and methylated H3K9
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IF
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mouse
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Citation
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