C10orf58 Mouse Monoclonal Antibody [7-B7]
Catalog# M1012-4
C10orf58 Mouse Monoclonal Antibody [7-B7]
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WB
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IF-Cell
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Human
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unconjugated
Overview
Product Name
C10orf58 Mouse Monoclonal Antibody [7-B7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human C10orf58 aa 1-229 / 229.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 26 kDa
Positive Control
Recombinant protein, human skin tissue lysate, HepG2, HUVEC, PC-3M.
Conjugation
unconjugated
Clone Number
7-B7
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:50-1:200
Target
Function
FAM213A, also known as PAMM (peroxiredoxin-like 2 activated in M-CSF stimulated monocytes) or C10orf58, is a 229 amino acid cytoplasmic protein that belongs to the peroxiredoxin-like FAM213 family and FAM213A subfamily. FAM213A involved in redox regulation of the cell. Acts as an antioxidant. Inhibits TNFSF11-induced NFKB1 and JUN activation and osteoclast differentiation. May affect bone resorption and help to maintain bone mass. Acts as a negative regulator of macrophage-mediated inflammation by inhibiting macrophage production of inflammatory cytokines, probably through suppression of the MAPK signaling pathway.
Background References
1. Chen YF et al. MicroRNA-211 Enhances the Oncogenicity of Carcinogen-Induced Oral Carcinoma by Repressing TCF12 and Increasing Antioxidant Activity. Cancer Res 76(16):4872-86 (2016).
2. Valverde G et al. A novel candidate region for genetic adaptation to high altitude in Andean populations.PLoS One 10(5):e0125444 (2015).
Sequence Similarity
Belongs to the peroxiredoxin-like PRXL2 family. PRXL2A subfamily.
Tissue Specificity
Expressed in CSF1 and TNFSF11-stimulated CD14(+) peripheral blood mononuclear cells (PBMCs).
Subcellular Location
Cytoplasm, Secreted.
UNIPROT
Synonyms
C10orf58 antibody
CJ058_HUMAN antibody
UPF0765 protein C10orf58 antibody
Images
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ICC staining C10orf58 (green) in HUVEC cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining C10orf58 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining C10orf58 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Western blot analysis of C10orf58 on different lysates with Mouse anti-C10orf58 antibody (M1012-4) at 1/2,000 dilution.
Lane 1: HUVEC (Human umbilical vein endothelial cell) cell lysate
Lane 2: SK-MEL-28 (Human melanoma cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 106 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: M1012-4, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
Predicted band size: 25 kDa
Observed band size: 25 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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