WSCD2 Mouse Monoclonal Antibody [9-F3]
Catalog# M1010-2
WSCD2 Mouse Monoclonal Antibody [9-F3]
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
WSCD2 Mouse Monoclonal Antibody [9-F3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein with Human WSCD2 50-353 / 565.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 64 kDa
Positive Control
WSCD2 recombinant protein lysates, mouse hippocampus tissue lysate, rat brain tissue lysate, SH-SY5Y, human thyroid tissue, human liver cancer tissue, human uterus tissue.
Conjugation
unconjugated
Clone Number
9-F3
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgM
Purification Method
Protein L affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:100-1:500
Target
Function
WSC domain-containing protein 2 (WSCD2) is a 565 amino acid, single-pass membrane protein that contains two WSC domains. The WSC domain is named after the yeast WSC1-WSC4 (cell wall integrity and stress response components 1-4) proteins, which each contain a WSC domain, and is a putative carbohydrate binding domain that spans about 90 amino acids. Containing eight conserved cysteine residues, which are predicted to form disulfide bridges, WSC domains are often found with other domains, such as LDL-receptor class A, PKD and C-type lectin. Two isoforms of WSCD2 exist as a result of alternative splicing events.
Background References
1. Tong SM et al. Subcellular localization of five singular WSC domain-containing proteins and their roles in Beauveria bassiana responses to stress cues and metal ions. Environ Microbiol Rep 8(2):295-304 (2016).
2. Gao C et al. A genome-wide linkage and association analysis of imputed insertions and deletions with cardiometabolic phenotypes in Mexican Americans: The Insulin Resistance Atherosclerosis Family Study. Genet Epidemiol 41(4):353-362 (2017).
Sequence Similarity
Belongs to the WSCD family.
Subcellular Location
Membrane.
Synonyms
4933413A10Rik antibody
C530024P05Rik antibody
Gm450 antibody
WSC domain containing 2 antibody
WSC domain containing protein 2 antibody
WSC domain-containing protein 2 antibody
WSCD 2 antibody
WSCD2 antibody
WSCD2_HUMAN antibody
Images
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Western blot analysis of WSCD2 on WSCD2 recombinant protein lysate using anti-WSCD2 antibody at 1/2000 dilution.
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Western blot analysis of WSCD2 on different lysates with Mouse anti-WSCD2 antibody (M1010-2) at 1/500 dilution.
Lane 1: Mouse hippocampus tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1010-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
ICC staining WSCD2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-WSCD2 antibody. Counter stained with
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Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-WSCD2 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-WSCD2 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"