FAM62B Mouse Monoclonal Antibody [C4-D0]
Catalog# M1009-1
FAM62B Mouse Monoclonal Antibody [C4-D0]
-
IHC-P
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
FAM62B Mouse Monoclonal Antibody [C4-D0]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human FAM62B aa 300-550.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC-P
Target Molecular Weight
Predicted band size: 102 kDa
Positive Control
Human colon cancer tissue, human kidney tissue.
Conjugation
unconjugated
Clone Number
C4-D0
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
-
IHC-P
-
1:200
Target
Function
Tethers the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane. Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport. Plays a role in FGF signaling via its role in the rapid internalization of FGFR1 that has been activated by FGF1 binding; this occurs most likely via the AP-2 complex. Promotes the localization of SACM1L at endoplasmic reticulum-plasma membrane contact sites (EPCS).
Background References
1. Min S.-W et. al. E-Syts, a family of membranous Ca2+-sensor proteins with multiple C2 domains. Proc. Natl. Acad. Sci. U.S.A. 104:3823-3828(2007).
Subcellular Location
Cell membrane, Endoplasmic reticulum, Membrane.
UNIPROT
Synonyms
Chr2 synaptotagmin antibody
CHR2SYT antibody
E Syt2 antibody
ESYT 2 antibody
ESYT2 antibody
Extended synaptotagmin 2 antibody
Extended synaptotagmin like protein 2 antibody
FAM 62B antibody
Family with sequence similarity 62 (C2 domain containing) member B antibody
Family with sequence similarity 62 member B antibody
ExpandImages
-
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-FAM62B antibody (M1009-1) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1009-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-FAM62B antibody (M1009-1) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1009-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"