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Transmembrane protein 200A Mouse Monoclonal Antibody [4-B3]

Usd: 350

Specification

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Catalog# M1008-8

Transmembrane protein 200A Mouse Monoclonal Antibody [4-B3]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

Transmembrane protein 200A Mouse Monoclonal Antibody [4-B3]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human Transmembrane protein 200A aa 59-359 / 491.

Species Reactivity

Human, Mouse

Validated Applications

WB, IF-Cell, IHC-P

Target Molecular Weight

Predicted band size: 54 kDa

Positive Control

HepG2, A549, F9, NCCIT, human pancreas tissue.

Conjugation

unconjugated

Clone Number

4-B3

RRID

AB_3073027

Product Features

Form

Liquid

Concentration

2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:50

Target

Function

Transmembrane protein 200A(TMEM200A) is multi-pass membrane protein, which belongs to the TMEM200 family. It is expressed in cerebellum.

Background References

1. Hui A B Y et al. Identification of a novel homozygous deletion region at 6q23.1 in medulloblastomas using high-resolution array comparative genomic hybridization analysis. Clin Cancer Res 11:4707-4716 (2005).

2. Carninci P et al. The transcriptional landscape of the mammalian genome. Science 309:1559-1563 (2005).

Sequence Similarity

Belongs to the TMEM200 family.

Tissue Specificity

Expressed in cerebellum.

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q86VY9 Human

SwissProt: Q8C817 Mouse

Synonyms

KIAA1913 antibody

T200A_HUMAN antibody

Tmem200a antibody

Transmembrane protein 200A antibody

TTMC antibody

Two transmembrane C antibody

Images

  • ICC staining Transmembrane protein 200A (red) in F9 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Transmembrane protein 200A (red) in F9 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Transmembrane protein 200A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Transmembrane protein 200A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Transmembrane protein 200A (red) in NCCIT cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Transmembrane protein 200A (red) in NCCIT cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Transmembrane protein 200A antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Transmembrane protein 200A antibody. Counter stained with hematoxylin.

  • Western blot analysis of Transmembrane protein 200A on different lysates with Mouse anti-Transmembrane protein 200A antibody (<a href="/products/M1008-8" style="font-weight: bold;text-decoration: underline;">M1008-8</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549(Human lung cancer cells) cell lysate <br />Lane 2: HepG2 (Human liver cancer cells) cell lysate <br />Lane 3: Caco-2 (Human colon adenocarcinoma cells) cell lysate <br />Lane 4: PC-3M (Human prostate cancer cells) cell lysate <br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 45 seconds; ECL: K1802<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/M1008-8" style="font-weight: bold;text-decoration: underline;">M1008-8</a>, 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Mouse IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 54.4 kDa<br />Observed band size: 47 kDa

    Western blot analysis of Transmembrane protein 200A on different lysates with Mouse anti-Transmembrane protein 200A antibody (M1008-8) at 1/1,000 dilution.

    Lane 1: A549(Human lung cancer cells) cell lysate
    Lane 2: HepG2 (Human liver cancer cells) cell lysate
    Lane 3: Caco-2 (Human colon adenocarcinoma cells) cell lysate
    Lane 4: PC-3M (Human prostate cancer cells) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 45 seconds; ECL: K1802

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: M1008-8, 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 54.4 kDa
    Observed band size: 47 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Alternative Products

metafield image

Transmembrane protein 200A Recombinant Mouse Monoclonal Antibody [4-B3-R]

Application: WB

Reactivity: Human

Conjugate: unconjugated