FAM3A Mouse Monoclonal Antibody [7-B9]
Catalog# M1008-10
FAM3A Mouse Monoclonal Antibody [7-B9]
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
FAM3A Mouse Monoclonal Antibody [7-B9]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human FAM3A 1-230 / 230.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC
Molecular Weight
Predicted band size: 25 kDa
Positive Control
Rat smooth muscle tissue lysates, human kidney tissue, human fetal skeletal muscle tissue, mouse heart tissue, A431.
Conjugation
unconjugated
Clone Number
7-B9
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:200
Target
Function
The FAM3 gene family is a novel cytokine-like gene family, which includes four members designated as FAM3A, FAM3B, FAM3C, and FAM3D. FAM3A is a secreted protein belonging to the FAM3 family of four alpha -helix bundle cytokines. It is expressed in multiple tissues. Mature human FAM3A shares approximately 93% amino acid sequence identity with the mouse and rat protein.FAM3A belongs to a novel cytokine-like gene family, and its physiological role remains largely unknown. FAM3A activated Akt through the p110α catalytic subunit of PI3K in an insulin-independent manner. Blockade of P2 ATP receptors or downstream phospholipase C (PLC) and IP3R and removal of medium calcium all significantly reduced FAM3A-induced increase in cytosolic free Ca2+ levels and attenuated FAM3A-mediated PI3K/Akt activation. Moreover, FAM3A-induced Akt activation was completely abolished by the inhibition of calmodulin (CaM).
Background References
1. Chunjiong W et al. FAM3A activates PI3K p110α/Akt signaling to ameliorate hepatic gluconeogenesis and lipogenesis. Hepatology 59(5):1779-90 (2014).
2. Jia S et al. FAM3A promotes vascular smooth muscle cell proliferation and migration and exacerbates neointima formation in rat artery after balloon injury. J Mol Cell Cardiol 74:173-82 (2014).
Sequence Similarity
Belongs to the FAM3 family.
Tissue Specificity
In similar amounts in testis, pancreas, adrenal, placenta, brain, fetal brain, liver, kidney, skeletal muscle and heart.
Subcellular Location
Secreted.
Synonyms
2-19 antibody
2.19 antibody
Cytokine-like protein 2-19 antibody
DLD antibody
DXS560S antibody
FAM3A antibody
FAM3A_HUMAN antibody
family with sequence similarity 3, member A antibody
Protein FAM3A antibody
XAP 7 antibody
Images
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Western blot analysis of FAM3A on rat smooth muscle tissue lysates with Mouse anti-FAM3A antibody (M1008-10) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 30 seconds;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1008-10) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FAM3A antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-FAM3A antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-FAM3A antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"