Synaptophysin Mouse Monoclonal Antibody [A1-7]
Catalog# M0407-30
Synaptophysin Mouse Monoclonal Antibody [A1-7]
-
WB
-
IF-Cell
-
IHC-P
-
FC
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
Synaptophysin Mouse Monoclonal Antibody [A1-7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide (KLH-coupled) within human Synaptophysin 220-280 aa.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
34kDa
Positive Control
Mouse brain tissue lysates, PANC-1,rat pancreas tissue, medullary thyroid carcinoma tissue, human pancreas tissue, mouse brain tissue, SHG-44.
Conjugation
unconjugated
Clone Number
A1-7
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:200-1:1,000
-
IF-Cell
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
Target
Function
Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms.
Background References
1. Janz R et al. Essential roles in synaptic plasticity for synaptogyrin I and synaptophysin I. Neuron 24:687-700 (1999).
2. Pekkurnaz G et al. Glucose regulates mitochondrial motility via Milton modification by O-GlcNAc transferase. Cell 158:54-68 (2014).
Sequence Similarity
Belongs to the synaptophysin/synaptobrevin family.
Tissue Specificity
Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3.
Post-translational Modification
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
Subcellular Location
Cytoplasmic vesicle. Membrane. Synapse. Synaptosome.
Synonyms
Major synaptic vesicle protein p38 antibody
MRX96 antibody
MRXSYP antibody
Syn p38 antibody
Synaptophysin antibody
Syp antibody
SYPH antibody
SYPH_HUMAN antibody
SypI antibody
Images
-
Western blot analysis of Synaptophysin on mouse brain tissue lysate using anti-Synaptophysin antibody at 1/2,000 dilution.
-
ICC staining Synaptophysin (red) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-Synaptophysin antibody (M0407-30) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0407-30) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded medullary thyroid carcinoma tissue with Mouse anti-Synaptophysin antibody (M0407-30) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0407-30) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Synaptophysin antibody (M0407-30) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0407-30) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Synaptophysin antibody (M0407-30) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0407-30) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Synaptophysin Recombinant Rabbit Monoclonal Antibody [JE42-51]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
