ATP5G1 Recombinant Rabbit Monoclonal Antibody [JE64-60]
Catalog# HA722921
ATP5G1 Recombinant Rabbit Monoclonal Antibody [JE64-60]
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WB
-
IHC-P
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Human
-
Mouse
-
Rat
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unconjugated
Overview
Product Name
ATP5G1 Recombinant Rabbit Monoclonal Antibody [JE64-60]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human ATP5G1 aa 51-100 / 136.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 14 kDa
Positive Control
HeLa cell lysate, Human liver tissue lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Rat liver tissue lysate, rat small intestine tissue.
Conjugation
unconjugated
Clone Number
JE64-60
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IHC-P
-
1:200
Target
Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.
Background References
1. Morciano G et al. A naturally occurring mutation in ATP synthase subunit c is associated with increased damage following hypoxia/reoxygenation in STEMI patients. Cell Rep. 2021 Apr.
2. Vives-Bauza C et al. Novel role of ATPase subunit C targeting peptides beyond mitochondrial protein import. Mol Biol Cell. 2010 Jan.
Subcellular Location
Mitochondrion membrane.
Synonyms
ATP synthase lipid-binding protein
ATP synthase membrane subunit c locus 1 Imported
ATP synthase proteolipid P1
ATP synthase proton-transporting mitochondrial F(0) complex subunit C1
ATPase protein 9
ATPase subunit c
Images
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Western blot analysis of ATP5G1 on different lysates with Rabbit anti-ATP5G1 antibody (HA722921) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: Human liver tissue lysate (20 µg/Lane)
Lane 3: Mouse liver tissue lysate (20 µg/Lane)
Lane 4: Mouse kidney tissue lysate (10 µg/Lane)
Lane 5: Rat liver tissue lysate (10 µg/Lane)
Predicted band size: 14 kDa
Observed band size: 8 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722921) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-ATP5G1 antibody (HA722921) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722921) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"