FADD Recombinant Rabbit Monoclonal Antibody [PSH07-78]
Catalog# HA722894
FADD Recombinant Rabbit Monoclonal Antibody [PSH07-78]
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WB
-
IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
FADD Recombinant Rabbit Monoclonal Antibody [PSH07-78]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse FADD aa 1-250.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 23 kDa
Positive Control
A431 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, mouse colon tissue, rat colon tissue.
Conjugation
unconjugated
Clone Number
PSH07-78
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
Target
Function
FAS-associated death domain protein, also called MORT1, is encoded by the FADD gene on the 11q13.3 region of chromosome 11 in humans. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development.
Background References
1. Liu Y et al. FADD as a key molecular player in cancer progression. Mol Med. 2022 Nov
2. Rodriguez DA et al. Caspase-8 and FADD prevent spontaneous ZBP1 expression and necroptosis. Proc Natl Acad Sci U S A. 2022 Oct
Subcellular Location
Cytoplasm, cytosol, plasma membrane, ripoptosome.
Synonyms
FADD antibody
FADD protein antibody
FADD_HUMAN antibody
Fas (TNFRSF6) associated via death domain antibody
Fas associated via death domain antibody
Fas associating death domain containing protein antibody
Fas associating protein antibody
Fas associating protein with death domain antibody
Fas TNFRSF6 associated via death domain antibody
FAS-associated death domain protein antibody
ExpandImages
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Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (HA722894) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: RAW264.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 27 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722894) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-FADD antibody (HA722894) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722894) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-FADD antibody (HA722894) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722894) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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FADD Recombinant Rabbit Monoclonal Antibody [PSH07-78] - BSA and Azide free
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Conjugate: unconjugated
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Conjugate: unconjugated
