MD2 Recombinant Rabbit Monoclonal Antibody [PSH07-72]
Catalog# HA722888
MD2 Recombinant Rabbit Monoclonal Antibody [PSH07-72]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MD2 Recombinant Rabbit Monoclonal Antibody [PSH07-72]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human MD2 aa 1-160.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 18 kDa
Positive Control
U-87 MG cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate.
Conjugation
unconjugated
Clone Number
PSH07-72
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Lymphocyte antigen 96, also known as "Myeloid Differentiation factor 2 (MD-2)," is a protein that in humans is encoded by the LY96 gene. The protein encoded by this gene is involved in binding lipopolysaccharide with Toll-Like Receptor (TLR4). The MD-2 protein appears to associate with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccharide (LPS), thus providing a link between the receptor and LPS signaling. That is, the primary interface between TLR4 and MD-2 is formed before binding LPS and the dimerization interface is induced by binding LPS.
Background References
1. Zhu W et al. Exercise-Induced Irisin Decreases Inflammation and Improves NAFLD by Competitive Binding with MD2. Cells. 2021 Nov
2. Li Y et al. Human MD2 deficiency-an inborn error of immunity with pleiotropic features. J Allergy Clin Immunol. 2023 Mar
Subcellular Location
Secreted, extracellular space.
Synonyms
ESOP 1 antibody
ESOP-1 antibody
ESOP1 antibody
LY 96 antibody
Ly-96 antibody
LY96 antibody
LY96_HUMAN antibody
Lymphocyte antigen 96 antibody
md 2 antibody
MD 2 protein antibody
ExpandImages
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Western blot analysis of MD2 on different lysates with Rabbit anti-MD2 antibody (HA722888) at 1/1,000 dilution.
Lane 1: U-87 MG cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: RAW264.7 cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Mouse kidney tissue lysate (40 µg/Lane)
Predicted band size: 18 kDa
Observed band size: 26 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722888) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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