CD44 Recombinant Rabbit Monoclonal Antibody [JE64-01] (HA722845)
Catalog# HA722845
Rabbit Monoclonal to CD44
Tested for
-
WB
-
IF-Cell
-
IHC-P
-
IF-Tissue
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD44 on different lysates with Rabbit anti-CD44 antibody (HA722845) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate (negative)
Lane 3: A549 cell lysate
Lane 4: Jurkat cell lysate (negative)
Lane 5: MDA-MB-231 cell lysate
Lane 6: LNCaP cell lysate (negative)
Lane 7: C2C12 cell lysate
Lane 8: J774A.1 cell lysate
Lane 9: RAW264.7 cell lysate
Lane 10: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 82 kDa
Observed band size: 82 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722845) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD44 on different lysates with Rabbit anti-CD44 antibody (HA722845) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate (negative)
Lane 3: A549 cell lysate
Lane 4: Jurkat cell lysate (negative)
Lane 5: MDA-MB-231 cell lysate
Lane 6: LNCaP cell lysate (negative)
Lane 7: C2C12 cell lysate
Lane 8: J774A.1 cell lysate
Lane 9: RAW264.7 cell lysate
Lane 10: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 82 kDa
Observed band size: 82 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722845) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.