Mono-Methyl Arginine (R*GG) Recombinant Rabbit Monoclonal Antibody [PSH07-52]
Catalog# HA722840
Mono-Methyl Arginine (R*GG) Recombinant Rabbit Monoclonal Antibody [PSH07-52]
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WB
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Species independent
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unconjugated
Overview
Product Name
Mono-Methyl Arginine (R*GG) Recombinant Rabbit Monoclonal Antibody [PSH07-52]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic mono-methyl arginine peptide library containing R*GG motifs.
Product Specificity
Mono-Methyl Arginine (R*GG) (PSH07-52) Rabbit mAb recognizes endogenous levels of proteins only when mono-methylated at arginine.
Species Reactivity
Species independent
Validated Applications
WB
Conjugation
unconjugated
Clone Number
PSH07-52
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
Target
Function
Methylation, in the chemical sciences, is the addition of a methyl group on a substrate, or the substitution of an atom (or group) by a methyl group. Methylation is a form of alkylation, with a methyl group replacing a hydrogen atom. These terms are commonly used in chemistry, biochemistry, soil science, and biology. In biological systems, methylation is catalyzed by enzymes; such methylation can be involved in modification of heavy metals, regulation of gene expression, regulation of protein function, and RNA processing. In vitro methylation of tissue samples is also a way to reduce some histological staining artifacts. The reverse of methylation is demethylation.
Background References
1. Mark T Bedford, Stéphane Richard. Arginine methylation an emerging regulator of protein function. Mol Cell. 2005 Apr 29;18(3):263-72.
2. Steffen Pahlich, Rouzanna P Zakaryan, Heinz Gehring. Protein arginine methylation: Cellular functions and methods of analysis. Biochim Biophys Acta. 2006 Dec;1764(12):1890-903.
Synonyms
Arginine (mono methyl) antibody
Mono-Methyl Arginine (R*GG)
Images
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☑ Cell treatment (CT)
Western blot analysis of Mono-Methyl Arginine (R*GG) on different lysates with Rabbit anti-Mono-Methyl Arginine (R*GG) antibody (HA722840) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: 293T treated with 10nM Type I PRMT inhibitor MS023 for 48 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Observed band size: Mutiple kDa
Exposure time: 5 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722840) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"