MSH3 Recombinant Rabbit Monoclonal Antibody [PSH07-41] (HA722829)
Catalog# HA722829
Rabbit Monoclonal to MSH3
Tested for
-
WB
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IF-Cell
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IHC-P
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of MSH3 on different lysates with Rabbit anti-MSH3 antibody (HA722829) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HUVEC cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: NCI-H1299 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: HCT 116 cell lysate (negative) (20 µg/Lane)
Lane 8: SW480 cell lysate (20 µg/Lane)
Lane 9: HepG2 cell lysate (20 µg/Lane)
Predicted band size: 127 kDa
Observed band size: 127 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722829) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of MSH3 on different lysates with Rabbit anti-MSH3 antibody (HA722829) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HUVEC cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: NCI-H1299 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: HCT 116 cell lysate (negative) (20 µg/Lane)
Lane 8: SW480 cell lysate (20 µg/Lane)
Lane 9: HepG2 cell lysate (20 µg/Lane)
Predicted band size: 127 kDa
Observed band size: 127 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722829) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.