Filamin B Recombinant Rabbit Monoclonal Antibody [PSH07-34]
Catalog# HA722822
Filamin B Recombinant Rabbit Monoclonal Antibody [PSH07-34]
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Filamin B Recombinant Rabbit Monoclonal Antibody [PSH07-34]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Filamin B aa
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 278 kDa
Positive Control
HeLa cell lysate, A549 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Rat lung tissue lysate, human colon tissue, rat colon tissue.
Conjugation
unconjugated
Clone Number
PSH07-34
Reactivity Data
| WB | IHC-P | IF-Tissue | |
|---|---|---|---|
| Human |
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|
| Mouse |
|
|
|
| Rat |
|
|
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
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IHC-P
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1:5,000
-
IF-Tissue
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1:1,000
Target
Function
Filamin B, beta (FLNB), also known as Filamin B, beta (truncated actin binding protein 278 homolog), is a cytoplasmic protein which in humans is encoded by the FLNB gene. FLNB regulates intracellular communication and signalling by cross-linking the protein actin to allow direct communication between the cell membrane and cytoskeletal network, to control and guide proper skeletal development. Mutations in the FLNB gene are involved in several lethal bone dysplasias, including boomerang dysplasia and atelosteogenesis type I.
Background References
1. Zhao X et al. Actin-binding protein filamin B regulates the cell-surface retention of endothelial sphingosine 1-phosphate receptor 1. J Biol Chem. 2023 Jul
2. Wu H et al. Cell-Dependent Pathogenic Roles of Filamin B in Different Skeletal Malformations. Oxid Med Cell Longev. 2022 Jul
Subcellular Location
Cytoplasm, cell cortex, cytoskeleton, stress, myofibril, sarcomere, Z line.
Synonyms
ABP 278 antibody
ABP 280 homolog antibody
ABP-278 antibody
ABP-280 homolog antibody
ABP278 antibody
Actin binding like protein antibody
Actin binding protein 278 antibody
Actin-binding-like protein antibody
AOI antibody
Beta filamin antibody
ExpandImages
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Western blot analysis of Filamin B on different lysates with Rabbit anti-Filamin B antibody (HA722822) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Rat lung tissue lysate (40 µg/Lane)
Predicted band size: 278 kDa
Observed band size: 315 kDa
Exposure time: Lane 1-3: 4 seconds; Lane 4-6: 42 seconds; ECL: K1801;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722822) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Filamin B antibody (HA722822) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722822) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Filamin B antibody (HA722822) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722822) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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