ERO1L Recombinant Rabbit Monoclonal Antibody [PSH07-30]
Catalog# HA722818
ERO1L Recombinant Rabbit Monoclonal Antibody [PSH07-30]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ERO1L Recombinant Rabbit Monoclonal Antibody [PSH07-30]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human ERO1L aa 1-468.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 54 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, L6 cell lysate, Mouse placenta tissue lysate, human colon tissue.
Conjugation
unconjugated
Clone Number
PSH07-30
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
Target
Function
ERO1-like protein alpha is a protein that in humans is encoded by the ERO1L gene. Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins 1. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.
Background References
1. Liu L et al. ERO1L Is a Novel and Potential Biomarker in Lung Adenocarcinoma and Shapes the Immune-Suppressive Tumor Microenvironment. Front Immunol. 2021 Jul
2. Xie J et al. ERO1L promotes the proliferation and metastasis of lung adenocarcinoma via the Wnt2/beta-catenin signaling pathway. Mol Carcinog. 2022 Oct
Subcellular Location
Endoplasmic reticulum membrane, membrane protein, Golgi apparatus lumen, Secreted, Cell projection, dendrite.
Synonyms
Endoplasmic oxidoreductin 1 like protein antibody
Endoplasmic oxidoreductin-1-like protein antibody
Endoplasmic reticulum oxidoreductase 1 alpha antibody
Endoplasmic reticulum oxidoreductin 1-like antibody
ERO1 alpha antibody
ERO1 L antibody
ERO1 Lalpha antibody
ERO1 like protein alpha antibody
ERO1-alpha antibody
ERO1-L antibody
ExpandImages
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Western blot analysis of ERO1L on different lysates with Rabbit anti-ERO1L antibody (HA722818) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: L6 cell lysate (20 µg/Lane)
Lane 6: Mouse placenta tissue lysate (40 µg/Lane)
Predicted band size: 54 kDa
Observed band size: 60 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722818) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ERO1L antibody (HA722818) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722818) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"