MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody [PSH07-24]
Catalog# HA722812
MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody [PSH07-24]
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WB
-
IHC-P
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IP
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Human
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unconjugated
Overview
Product Name
MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody [PSH07-24]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human MAP4K1 aa 301-500.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
Predicted band size: 91 kDa
Positive Control
Jurkat cell lysate, Raji cell lysate, Ramos cell lysate, Daudi cell lysate, THP-1 cell lysate, human lymph node tissue, human spleen tissue.
Conjugation
unconjugated
Clone Number
PSH07-24
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:2,000
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IHC-P
-
1:2,000
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IP
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1-2μg/sample
Target
Function
Mitogen-activated protein kinase kinase kinase kinase 1 is a protein kinase that in humans is encoded by the MAP4K1 gene. It is also known as HPK1 (Hematopoietic Progenitor Kinase 1). The protein has been shown to play a role in JNK activation.
Background References
1. Sun JM et al. Glioblastoma cellular MAP4K1 facilitates tumor growth and disrupts T effector cell infiltration. Life Sci Alliance. 2023 Sep
2. He TS et al. The Kinase MAP4K1 Inhibits Cytosolic RNA-Induced Antiviral Signaling by Promoting Proteasomal Degradation of TBK1/IKKepsilon. Microbiol Spectr. 2021 Dec
Subcellular Location
Cytoplasm, membrane.
UNIPROT
Synonyms
Hematopoietic progenitor kinase 1 antibody
Hematopoietic progenitor kinase antibody
HPK1 antibody
Human Hematopoietic Progenitor Kinase antibody
M4K1_HUMAN antibody
Map4k1 antibody
MAPK/ERK kinase kinase kinase 1 antibody
MEK kinase kinase 1 antibody
MEKKK 1 antibody
Mitogen activated protein kinase kinase kinase kinase 1 antibody
ExpandImages
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Western blot analysis of MAP4K1 / HPK1 on different lysates with Rabbit anti-MAP4K1 / HPK1 antibody (HA722812) at 1/2,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: Raji cell lysate (20 µg/Lane)
Lane 3: Ramos cell lysate (20 µg/Lane)
Lane 4: Daudi cell lysate (20 µg/Lane)
Lane 5: THP-1 cell lysate (20 µg/Lane)
Predicted band size: 91 kDa
Observed band size: 97 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722812) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-MAP4K1 / HPK1 antibody (HA722812) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722812) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-MAP4K1 / HPK1 antibody (HA722812) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722812) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
MAP4K1 / HPK1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722812 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722812 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (input)
Lane 2: HA722812 IP in Jurkat cell lysate
Lane 3: Rabbit IgG instead of HA722812 in Jurkat cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 8 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody [PSH07-24] - BSA and Azide free
Application: WB,IHC-P,IP
Reactivity: Human
Conjugate: unconjugated
